...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A cytochrome b_5 is required for full activity of flavonoid 3',5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors
【24h】

A cytochrome b_5 is required for full activity of flavonoid 3',5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors

机译：类黄酮3'，5'-羟化酶的全部活性需要细胞色素b_5，这是一种参与形成蓝色花朵颜色的细胞色素P450

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The substitution pattern of anthocyanin pig- ments is a main determinant of flower color. Flavonoid 3',5'-hydroxylase (F3'5'H) is a cytochrome P450 enzyme (Cyt P450) that catalyzes the 3',5'-hydroxylation of dihydrofla- vonols, the precursors of purple anthocyanins. Species such as rose and carnation lack F3'5'H activity and are, therefore, unable to generate purple or blue flowers. Petunia, on the other hand, contains two loci, termed hfl and hf2, that encode a Cyt P450 with F3'5'H activity. Here we report the identifi- cation of an additional petunia gene that is required for 3',5' substitution of anthocyanins and purple flower colors. It encodes a cytocbrome b_5 and is expressed exclusively in the flower. Inactivation of the genc by targeted transposon mu- tagenesis reduced F3'5'H enzyme activity and the accumula- tion of 5'-substituted anthocyanins, resulting in an altered flower color. However, no phenotypic effect on the activity of other Cyt P450s, involved in the synthesis of hormones or general phenylpropanoids, was observed. These data provide in vivo evidence for the regulation of the activity of specific Cyt P450s by a cytochrome b_5.
机译：花色苷色素的取代模式是决定花朵颜色的主要因素。类黄酮3'，5'-羟化酶（F3'5'H）是一种细胞色素P450酶（Cyt P450），它催化紫色花青素的前体二氢黄酮酚的3'，5'-羟基化。玫瑰和康乃馨等物种缺乏F3'5'H活性，因此无法生成紫色或蓝色的花朵。另一方面，矮牵牛含有称为hf1和hf2的两个基因座，它们编码具有F3'5'H活性的Cyt P450。在这里，我们报告鉴定出花青素和紫色花色的3'，5'替代所需要的另一个矮牵牛基因。它编码细胞核蛋白组b_5，仅在花中表达。靶向转座子诱变使基因失活会降低F3'5'H酶的活性和5'-取代的花色苷的积累，从而导致花色的改变。但是，未观察到对参与激素或一般苯基丙烷合成的其他Cyt P450活性的表型影响。这些数据提供了通过细胞色素b_5调节特定Cyt P450活性的体内证据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号