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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Four intracellular genomes direct weevil biology: Nuclear, mitochondrial, principal endospobiont, and Wolbachia
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Four intracellular genomes direct weevil biology: Nuclear, mitochondrial, principal endospobiont, and Wolbachia

机译：四个细胞内基因组指导象鼻虫生物学：核，线粒体，主要内生孢子虫和沃尔巴克氏菌

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Cell physiology in the weevil Sitophilus oryzae is coordinated by three integrated genomes: nuclear, mito- chondrial, and the "S. oryzae principal endosymbiont" (SOPE). SOPE, a cytoplasmic bacterium (2 x 10~3 bacteria per specialized bacteriocyte cell and 3 x 10~6 bacteria per weevil) that belongs to the proteobacteria #gamma#3-subgroup, is present in all weevils studied. We discovered a fourth prokaryotic ge- nome in somatic and germ tissues of 57 of weevil strains of three species, S. oryzae, Sitophilus zeamais, and Sitophilus granarius, distributed worldwide. We assigned this Gram- negative prokaryote to the Wolbachia group (#alpha#-proteobacte- ria), on the basis of 16S rDNA sequence and fluorescence in situ DNA-RNA hybridization (FISH). Both bacteria, SOPE and Wolbachia, were selectively eliminated by combined heat and antibiotic treatments. Study of bacteria involvement in this insect's genetics and physiology revealed that SOPE, which induces the specific differentiation of the bacteriocytes, increases mitochondrial oxidative phosphorylation through the supply of pantothenic acid and riboflavin. Elimination of this #gamma#3-proteobacterium impairs many physiological traits. By contrast, neither the presence nor the absence of Wolbachia significantly affects the weevil's physiology. Wolbachia, dis- seminated throughout the body cells, is in particularly high density in the germ cells, where it causes nucleocytoplasmic incompatibility. The coexistence of two distinct types of in- tracellular proteobacteria at different levels of symbiont integration in insects illustr
机译：象鼻Sitophilus oryzae的细胞生理由三个完整的基因组协调：核，线粒体和“ S. oryzae主要内生共生体”（SOPE）。 SOPE是一种属于细菌＃gamma＃3-子群的胞质细菌（每个专门的细菌细胞细胞2 x 10〜3个细菌，每个象鼻虫3 x 10〜6个细菌），存在于所有研究的象鼻虫中。我们在分布于世界各地的3种米曲霉，米黄色葡萄球菌，玉米白粉虱和细叶Sitophilus granarius的57种象鼻虫的体细胞和生殖组织中发现了第四个原核基因组。我们根据16S rDNA序列和荧光原位DNA-RNA杂交（FISH），将该革兰氏阴性原核生物分配给Wolbachia组（＃alpha＃-proteobacteria）。 SOPE和Wolbachia两种细菌均通过加热和抗生素联合处理而被选择性清除。对细菌参与该昆虫的遗传和生理的研究表明，SOPE可以诱导细菌细胞的特异性分化，并通过提供泛酸和核黄素来增加线粒体的氧化磷酸化作用。消除这种＃γ＃3-变形杆菌会损害许多生理特性。相比之下，沃尔巴赫菌的存在与否都不会显着影响象鼻虫的生理。遍布全身细胞的沃尔巴氏菌在生殖细胞中的密度特别高，会导致核质不相容。昆虫说明中共生水平不同的两种不同类型的细胞内蛋白细菌的共存

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