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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Feedback control of a master bacterial cell-cycle regulator
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Feedback control of a master bacterial cell-cycle regulator

机译：主细菌细胞周期调节器的反馈控制

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The transcriptional regulator CtrA controls several key cell-cycle events in Caulobacter crescentus, includ- ing the initiation of DNA replication, DNA methylation, cell division, and flagellar biogenesis. CtrA is a member of the response regulator family of two component signal transduc- tion systems. Caulobacter goes to great lengths to control the time and place of the activity of this critical regulatory factor during the cell cycle. These controls include temporally reg- ulated transcription and phosphorylation and spatially re- stricted proteolysis. We report here that ctrA expression is under the control of two promooters: a promoter (P1) that is active only in the early predivisional cell and a stronger promoter (P2) that is active in the late predivisional cell. Both promoters exhibit CtrA-mediated feedback regulation: the early P1 promoter is negatively controlled by CtrA and the late P2 promoter is under positive feedback control. The CtrA protein footprints conserved binding sites within the P1 and P2 promoters. We propose that the P1 promoter is activated after the initiation of DNA replication in the early predivi- sional cell. The ensuing accumulation of CtrA results in the activation of the P2 promoter and the repression of the P1 promoter late in the cell cycle. Thus, two transcriptional feedback loops coupled to cell cycle-regulated proteolysis and phosphorylation of the CtrA protein result in the pattern of CtrA activity required for the temporal and spatial control of multiple cell-cycle events.
机译：转录调节因子CtrA控制新月形杆菌中的几个关键细胞周期事件，包括DNA复制，DNA甲基化，细胞分裂和鞭毛生物发生的启动。 CtrA是两个成分信号转导系统的响应调节器系列的成员。杆状杆菌竭尽全力在细胞周期中控制该关键调节因子的活性的时间和位置。这些控制包括时间调控的转录和磷酸化以及空间受限的蛋白水解。我们在这里报告ctrA表达受两个启动子的控制：一个启动子（P1）仅在早期preprevisional细胞中有活性，而一个较强的启动子（P2）在晚期preprevisional细胞中有活性。这两个启动子均表现出CtrA介导的反馈调节：早期的P1启动子受CtrA负控制，而晚期的P2启动子则受正反馈控制。 CtrA蛋白足迹保留P1和P2启动子内的结合位点。我们建议在早期分裂细胞中启动DNA复制后，激活P1启动子。随后的CtrA积累导致P2启动子的活化和细胞周期后期P1启动子的阻遏。因此，与细胞周期调节的蛋白水解和CtrA蛋白的磷酸化偶联的两个转录反馈环导致了对多个细胞周期事件进行时间和空间控制所需的CtrA活性模式。

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