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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Map-based cloning of chloronerva, a gene involved in iron uptake of higher plants encoding nicotianamine synthase
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Map-based cloning of chloronerva, a gene involved in iron uptake of higher plants encoding nicotianamine synthase

机译：基于图的克隆氯神经的克隆，氯神经是一个基因，其参与编码烟碱胺合酶的高等植物的铁吸收

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摘要

The uptake of iron in plants is a highly regulated process that is induced on iron starvation. In tomato, the mutant chloronerva exhibits constitutive expres- sion of iron uptake responses and intercostal chlorosis. Biochemically, chloronerva is an auxotroph for nicotianamine, a key polyamine in plant iron uptake metabolism. The chlo- ronerva gene has been fine-mapped onto the long arm of chromosome 1 in a large segregating tomato population and yeast artificial chromosome clones encompassing the region were isolated by using flanking markers. A cosmid contig containing the chloronerva gene was established, and comple- menting cosmids were identified by transformation into the mutant. The chloronerva transcript was identified by cDNA isolation using the complementing cosmids. The gene encodes a unique protein of 35 kDa. The mutant harbors a single base change compared with the wild type. Based on enzyme activity and sequence similarity to the coding DNA sequence of the purified barley enzyme the chloronerva gene encodes the enzyme nicotianamine synthase.
机译：植物中铁的吸收是铁饥饿导致的高度调控的过程。在番茄中，突变型的氯代神经显示出铁吸收反应和肋间萎黄的组成型表达。从生化角度看，氯神经氨酸是烟碱胺的营养缺陷型，后者是植物铁吸收代谢中的关键多胺。 chronronva基因已被精细映射到一个较大的分离番茄种群的1号染色体的长臂上，并使用侧翼标记分离了包围该区域的酵母人工染色体克隆。建立了含有氯神经氨酸基因的粘粒重叠群，并通过转化为突变体来鉴定互补粘粒。通过使用互补粘粒的cDNA分离来鉴定氯神经转录物。该基因编码35 kDa的独特蛋白质。与野生型相比，该突变体具有单个碱基变化。基于酶活性和与纯化的大麦酶的编码DNA序列的序列相似性，氯神经基因编码酶烟碱胺合酶。

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