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首页> 外文期刊>Process Biochemistry >Optimal production of sulphuric acid by Thiobacillus thiooxidans using response surface methodology
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Optimal production of sulphuric acid by Thiobacillus thiooxidans using response surface methodology

机译：响应面分析法优化硫代氧化硫杆菌的硫酸生产

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摘要

Response surface methodology was applied to determine the optimal medium composition for sulphuric acid production by an indigenous Thiobacillus thiooxidans. With a view to reducing the number of experiments and obtaining the mutual interactions between the variables, a 2~4 full-factorial central composite design was employed for experimental design and analysis of the results. Four independent variables, KH_2PO_4, (NH_4)_2SO_4, MgSO_4 and elemental sulphur, in the growth medium were tested. Elemental sulphur was found to exhibit positive correlation with the sulphuric acid yield, whereas the other three variables showed no significant effect. No mutual interactions were found between elemental sulphur and each of the other three variables. The optimal composition of the growth medium to achieve the optimal production of sulphuric acid was determined as follows (g/l): KH_2PO_4 = 3.5, (NH_4)_2SO_4 = 4.9, MgSO_4 = 0.7 and elemental sulphur = 23.7. The corresponding sulphuric acid production and dry cell weight were 38 662 ppm and 0.7 g/l, which are about 2.6- and 1.75-fold increase, respectively, compared with those using the conventional T. thiooxidans optimum growth medium.
机译：应用响应面方法确定本地硫代硫氧硫杆菌生产硫酸的最佳培养基组成。为了减少实验次数并获得变量之间的相互影响，采用2〜4个全因子中心复合设计进行实验设计和结果分析。测试了生长培养基中的四个独立变量KH_2PO_4，（NH_4）_2SO_4，MgSO_4和元素硫。发现元素硫与硫酸产率呈正相关，而其他三个变量则无显着影响。在元素硫与其他三个变量之间没有发现相互作用。确定实现硫酸最佳生产的生长培养基的最佳组成如下（g / l）：KH_2PO_4 = 3.5，（NH_4）_2SO_4 = 4.9，MgSO_4 = 0.7和元素硫= 23.7。与使用常规硫代氧化硫最佳生长培养基的硫酸盐产量和干细胞重量相比，相应的硫酸产量和干细胞重量分别为38 662 ppm和0.7 g / l，分别提高了约2.6和1.75倍。

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