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首页> 外文期刊>Process Biochemistry >Effects of Na_2SeO_3 on growth, metabolism, antioxidase and enzymes involved in polysaccharide synthesis of Cordyceps militaris
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Effects of Na_2SeO_3 on growth, metabolism, antioxidase and enzymes involved in polysaccharide synthesis of Cordyceps militaris

机译：Na_2SeO_3对蛹虫草多糖合成参与多糖合成的生长，代谢，抗氧化酶和酶的影响

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摘要

The main objective of our study was to illuminate effects of sodium selenite (Na2SeO3) on growth, metabolism and enzyme activities of Cordyceps militaris. By adding Na2SeO3 in fermentation medium (7 mg/L), it was found that mycelium of C. militaris was stronger with more plump spores and its biomass was higher accompanied by the maximum (13.19 g/L) at 6 d of culture. Besides, Na2SeO3 also caused the enhancement of total thiol (T-SH), non-protein thiol (NP-SH) contents and the biosynthesis of Se-polysaccharide (Se-CMP) with discriminatory molecular weight, optical rotation, UV-vis and FT-IR spectra. Activities of antioxidase including catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and enzymes involved in polysaccharide synthesis including phosphoglucomutase (PGM), phosphoglucose isomerase (PGI), UDPG-pyrophosphorylase (UGP) enhanced to different extent. Their corresponding genes were also up-regulated but cat gene (encoding CAT). Se enrichment of C. militaris provided a good way for desirable biomass and polysaccharide biosynthesis in further research.
机译：我们研究的主要目的是阐明硒（Na2SeO3）对冬虫夏草军团生长，代谢和酶活性的影响。通过在发酵培养基（7mg / L）中加入Na 2 Se 3，发现C.Militaris的菌丝体较强，孢子率更强，其生物质伴随着6d培养物的最大（13.19g / L）。此外，Na2SeO3还导致增强总硫醇（T-SH），非蛋白质硫醇（NP-SH）含量和Se-多糖（Se-CMP）的生物合成，具有辨别分子量，光学旋转，UV-Vis和FT-IR光谱。包括过氧化氢酶（CAT），超氧化物歧化酶（SOD），谷胱甘肽过氧化物酶（GSH-PX）和参与多糖合成的酶，包括磷光血糖酶（PGM），磷光葡萄糖异构酶（PGI），UDPG-焦磷酸化酶（UGP）的酶，增强了不同程度。它们的相应基因也上调，但猫基因（编码猫）。富集C. MILITARIS为进一步研究中的理想生物质和多糖生物合成提供了一种很好的方法。

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  • 来源
    《Process Biochemistry》 |2020年第10期|64-71|共8页
  • 作者

    Ren Yuan-yuan; Sun Peng-peng; Li Hong-ran; Zhu Zhen-yuan;

  • 作者单位

    Yangtze Univ Coll Life Sci Jingzhou 434023 Hubei Peoples R China|Tianjin Univ Sci & Technol State Key Lab Food Nutr & Safety Tianjin 300457 Peoples R China|Tianjin Univ Sci & Technol Key Lab Food Nutr & Safety Minist Educ Tianjin 300457 Peoples R China|Tianjin Univ Sci & Technol Coll Food Sci & Biotechnol Tianjin 300457 Peoples R China;

    Yangtze Univ Coll Life Sci Jingzhou 434023 Hubei Peoples R China;

    Tianjin Univ Sci & Technol State Key Lab Food Nutr & Safety Tianjin 300457 Peoples R China|Tianjin Univ Sci & Technol Key Lab Food Nutr & Safety Minist Educ Tianjin 300457 Peoples R China|Tianjin Univ Sci & Technol Coll Food Sci & Biotechnol Tianjin 300457 Peoples R China;

    Tianjin Univ Sci & Technol State Key Lab Food Nutr & Safety Tianjin 300457 Peoples R China|Tianjin Univ Sci & Technol Key Lab Food Nutr & Safety Minist Educ Tianjin 300457 Peoples R China|Tianjin Univ Sci & Technol Coll Food Sci & Biotechnol Tianjin 300457 Peoples R China;

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  • 正文语种 eng
  • 中图分类
  • 关键词

    Cordyceps militaris; Sodium selenite; Enzymes; Polysaccharide; Thiol;

    机译：冬虫夏草军团;亚硒酸钠;酶;多糖;硫醇;

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