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首页> 外文期刊>Protein and Peptide Letters >Spectroscopic Studies on Erythrina lysistemon Seed Lectin Effect of Denaturing Agents on Lectin Quaternary Structure
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Spectroscopic Studies on Erythrina lysistemon Seed Lectin Effect of Denaturing Agents on Lectin Quaternary Structure

机译：溶血赤藓菌种子凝集素变性剂对凝集素第四级结构的光谱研究

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摘要

The equilibrium unfolding of ElysL, a homodimeric legume lectin, was studied using different denaturing agents such as guanidinium chloride (GdnHCl), temperature and pH. Simultaneously, changes in the secondary as well as tertiary structure of lectin were followed by CD spectroscopy examination in both far and near-UV region, respectively. The hydrophobic cluster binding dye, 1-anilino-8-naphthalene sulfonate (ANS), was used to further explore intermediates and to follow the unfolding pathway of lectin. The adenine binding ability of lectin was examined and monitored via absorption spectra and the intrinsic tryptophan fluorescence. Our findings indicate that the ElysL unfolding process occurs via a three state pathway with an intermediate state. We also showed that ElysL binds adenine in a manner that involves a hydrophobic binding pocket that is independent of the carbohydrate binding sites.
机译：使用不同的变性剂，例如氯化胍（GdnHCl），温度和pH，研究了同型二聚体豆类植物凝集素ElysL的平衡展开。同时，在远紫外区和近紫外区分别通过CD光谱法检查凝集素的二级和三级结构的变化。疏水性簇结合染料1-苯胺基-8-萘磺酸盐（ANS）用于进一步探索中间体并遵循凝集素的展开途径。通过吸收光谱和固有色氨酸荧光检查和监测凝集素的腺嘌呤结合能力。我们的发现表明ElysL的展开过程是通过具有中间状态的三态途径发生的。我们还显示，ElysL以涉及疏水结合袋的方式结合腺嘌呤，该结合袋独立于碳水化合物结合位点。

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