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首页> 外文期刊>Radiation Protection Dosimetry >BIOLOGICAL MARKERS OF EXTERNAL AND INTERNAL EXPOSURE IN SHELTER CONSTRUCTION WORKERS: A 13-YEAR EXPERIENCE
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BIOLOGICAL MARKERS OF EXTERNAL AND INTERNAL EXPOSURE IN SHELTER CONSTRUCTION WORKERS: A 13-YEAR EXPERIENCE

机译：棚架建筑工人外部和内部暴露的生物标志：13年的经验

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Objective: The aim of this study is to investigate the cellular, molecular, genetic and non-invasive functional biomarkers of occupational irradiation in workers exposed to a combination of external gamma-radiation and incorporation of transuranium elements. Results: A study was performed in 688 radiation workers of Shelter object conversion into a safe system with mean shift dose of external exposure of 26.06 mSv (range: 0.1-113.35) and risk of internal exposure with transuranium elements. Several biological parameters could serve as biomarkers of exposure at radiation doses below 100 mSv and even in 20-50 mSv interval. The parallel changes were shown in decline of brain electric activity, telomere length, differences in CCND1, CDKN1A, CDKN2A, VEGFA, TP53, DDB2 genes expression. An increase in counts of dicentrics, pair fragments and TCR-variant lymphocytes at doses over professional limits shows the need of biological dosimetry. The most sensitive markers include TCR-CD4+, gamma-H2AX+ and CyclinD1+ cell counts. Implementation of flow cytometry approach for these markers enables quick obtaining of quantitative data. Confounding factors included respiratory function and smoking. The study of the radiation workers with the history of chronic exposure in radiation area during 3-5 years demonstrates changes of compensatory origin, i. e. absence of telomere shortening, increased number of NK-cells in combination with lower expression of intracellular gamma-H2AX. Conclusion: This study confirms the presence of radiation-induced changes in gene regulation of cell proliferation, telomere function and apoptosis in radiation workers exposed to external and internal exposure at doses above professional limits and increase of compensatory changes.
机译：目的：本研究的目的是研究暴露于外部伽马射线和掺入铀元素的工人的职业照射的细胞，分子，遗传和非侵入性功能性生物标志物。结果：进行了一项研究，研究对象是688名放射防护人员，他们将避难所物体转换为安全系统，外部转移的平均移动剂量为26.06 mSv（范围：0.1-113.35），并且内部暴露有铀元素的风险。在低于100 mSv的辐射剂量下，甚至在20-50 mSv的间隔内，几种生物学参数都可以作为暴露的生物标志。平行变化显示为脑电活动的下降，端粒长度，CCND1，CDKN1A，CDKN2A，VEGFA，TP53，DDB2基因表达的差异。剂量超过专业极限时，双着丝粒，对片段和TCR变异淋巴细胞的计数增加，表明需要进行生物剂量测定。最敏感的标记包括TCR-CD4 +，γ-H2AX+和CyclinD1 +细胞计数。这些标记的流式细胞仪方法的实施使得能够快速获得定量数据。混杂因素包括呼吸功能和吸烟。辐射工作人员在3-5年内有辐射区域慢性暴露史的研究表明，补偿源的变化，即。 e。不存在端粒缩短，NK细胞数量增加以及细胞内γ-H2AX表达降低的情况。结论：这项研究证实了辐射诱发的细胞增殖，端粒功能和细胞凋亡的基因调控存在于暴露剂量超过专业限制和补偿性变化增加的外部和内部暴露的辐射工作者中。

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