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首页> 外文期刊>Reactive & Functional Polymers >Epoxy functionalized polymer grafted magnetic nanoparticles by facile surface initiated polymerization for immobilization studies of Candida Antarctica lipase B
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Epoxy functionalized polymer grafted magnetic nanoparticles by facile surface initiated polymerization for immobilization studies of Candida Antarctica lipase B

机译：易表面引发的环氧官能化聚合物接枝磁性纳米粒子的固定化研究南极假丝酵母脂肪酶B

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摘要

Immobilization of Candida Antarctica lipase B (Cal-B) was done on the epoxy functionalized polymer (EFP) grafted magnetic nanoparticles (MNPs) via covalent attachment with the active epoxy groups. The EFP brushes were grafted on iron-oxide based MNPs by a facile surface-initiated atom transfer radical polymerization (ATRP) using activators generated by electron transfer (AGET) of glycidyl methacrylate (GMA). Each step of the surface modification, polymer grafting, and enzyme immobilization process on the polymer grafted MNPs was studied using Fourier transform infrared spectroscopy (FTIR). A thermogravimetric analysis (TGA) calculated the amount of engineered organic components, a transmission electron microscopy (TEM) visualized the core-shell formation of the MNPs, and a vibrating sample magnetometer (VSM) validated their magnetic properties at various modification stages. The lipase immobilization efficiency was described as a function of immobilization time, as well as, enzyme amount. The activity was characterized within a range of pH, temperature, kinetic parameters, resusability and storage stability, for both the free and immoblized Cal-B enzyme. The results of this study suggested that poly(GMA) grafted MNPs can be successfully used for the immobilization of Cal-B with improved efficiencies compared to those obtained with free soluble lipase. The reported enzyme immobilization method appears to be reproducible and scalable for industrial production.
机译：南极假丝酵母脂肪酶B（Cal-B）的固定是通过与活性环氧基团的共价连接在环氧官能化聚合物（EFP）接枝的磁性纳米颗粒（MNP）上完成的。通过使用由甲基丙烯酸缩水甘油酯（GMA）的电子转移（AGET）生成的活化剂，通过表面引发的原子转移自由基聚合（ATRP）轻松地将EFP刷子接枝到基于氧化铁的MNP上。使用傅立叶变换红外光谱（FTIR）研究了在聚合物接枝的MNP上进行表面修饰，聚合物接枝和酶固定过程的每个步骤。热重分析（TGA）计算了工程有机成分的量，透射电子显微镜（TEM）可视化了MNP的核壳形成，振动样品磁力计（VSM）验证了它们在各个修饰阶段的磁性能。脂肪酶的固定效率被描述为固定时间以及酶量的函数。对于游离的和固定化的Cal-B酶，在pH，温度，动力学参数，重用性和储存稳定性的范围内表征活性。这项研究的结果表明，与游离可溶性脂肪酶相比，聚（GMA）接枝的MNPs可以成功地用于固定Cal-B，具有更高的效率。报道的酶固定方法似乎可重现且可扩展用于工业生产。

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  • 来源
    《Reactive & Functional Polymers》 |2020年第2期|104454.1-104454.8|共8页
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  • 作者单位

    King Faisal Univ Coll Clin Pharm Dept Pharm Practice Al Hasa Saudi Arabia;

    CSIR Natl Chem Lab Chem Engn & Proc Dev Div Pune 411008 Maharashtra India;

    King Faisal Univ Coll Clin Pharm Dept Pharmaceut Sci Al Hasa Saudi Arabia;

    King Faisal Univ Coll Med Dept Biomed Sci Al Hasa Saudi Arabia;

    King Faisal Univ Coll Clin Pharm Dept Biomed Sci Al Hasa Saudi Arabia;

    Govt Coll Pharm Dept Pharmacognosy Bengaluru India;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Immoblization; Cal-B enzymes; Magnetic nanoparticles; Enzymes;

    机译：固定;Cal-B酶;磁性纳米粒子;酵素;

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