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首页> 外文期刊>Reactive & Functional Polymers >Preparation of a surface-grafted protein-selective polymer film by combined use of controlled/living radical photopolymerization and microcontact imprinting
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Preparation of a surface-grafted protein-selective polymer film by combined use of controlled/living radical photopolymerization and microcontact imprinting

机译：通过控制/活性自由基光聚合和微接触压印的结合使用来制备表面接枝的蛋白质选择性聚合物膜

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摘要

In this study, we describe a strategy for the formation of a molecularly imprinted polymer (MIP) capable of selective rebinding of protein-sized molecules and interfaced with a planar sensing surface. The strategy is based on the synergistic use of the surface-initiated controlled/living radical (C/LR) photopolymerization and microcontact imprinting approach aiming at design of a protein-responsive polymer for biosensing application. Bovine serum albumin (BSA), 2-(diethylamino)ethyl methacrylate, bis-acrylamide were used as a model protein, a functional monomer and a cross-linker, respectively, to prepare the BSA-MIP film. The optimal parameters of C/LR photopolymerization such as the method for photoinitiator attachment to the sensor surface, monomencross-linker molar ratio, polymerization time, were determined. The BSA-MIP film were studied in terms of their recognition capability and selectivity towards the target protein (BSA) through the analysis of the responses of the BSA-MIP modified SPR sensors upon interaction with BSA and interfering proteins, human serum albumin (HSA) and Pc-fragment of immunoglobulin G (Fc). It was found that BSA-MIP adsorbed BSA with the dissociation constant (RD) in the nanomolar range (68 nM) and shows more than two times higher adsorption capacity as compared to HSA and Fc, even though their molecular sizes were similar. Also, BSA-MIP could be perfectly regenerated in the alkaline solution showing nearly reversible responses (loss of 2.4%) even after the 25th regeneration cycle. The presented simple synthesis strategy could be potentially employed for the preparation of protein-MT films on a planar sensor transducer allowing to develop sensing systems for detection of clinically relevant proteins.
机译：在这项研究中，我们描述了一种形成分子印迹聚合物（MIP）的策略，该分子印迹聚合物能够选择性地重新结合蛋白大小的分子并与平面感测表面连接。该策略基于表面引发的受控/活性自由基（C / LR）光聚合和微接触压印方法的协同使用，旨在设计用于生物传感应用的蛋白质响应聚合物。牛血清白蛋白（BSA），甲基丙烯酸2-（二乙氨基）乙酯，双丙烯酰胺分别用作模型蛋白，功能单体和交联剂，以制备BSA-MIP膜。确定了C / LR光聚合反应的最佳参数，例如光引发剂附着在传感器表面的方法，单交联剂摩尔比，聚合时间。通过分析BSA-MIP修饰的SPR传感器与BSA和干扰蛋白，人血清白蛋白（HSA）相互作用后的响应，研究了BSA-MIP膜的识别能力和对目标蛋白（BSA）的选择性。和免疫球蛋白G（Fc）的Pc片段。已发现，BSA-MIP吸附的BSA的解离常数（RD）在纳摩尔量（68 nM）范围内，并且与HSA和Fc相比，其吸附容量高出两倍以上，即使它们的分子大小相似。同样，即使在第25次再生循环之后，BSA-MIP也可以在碱性溶液中完美再生，显示出几乎可逆的响应（损失2.4％）。提出的简单合成策略可潜在地用于在平面传感器换能器上制备蛋白质MT膜，从而允许开发用于检测临床相关蛋白质的传感系统。

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