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首页> 外文期刊>Rejuvenation Research >Response to Histamine Allows the Functional Identification of Neuronal Progenitors, Neurons, Astrocytes, and Immature Cells in Subventricular Zone Cell Cultures
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Response to Histamine Allows the Functional Identification of Neuronal Progenitors, Neurons, Astrocytes, and Immature Cells in Subventricular Zone Cell Cultures

机译:对组胺的反应允许在脑室下区细胞培养物中对神经元祖细胞,神经元,星形胶质细胞和未成熟细胞进行功能鉴定

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摘要

Subventricular zone (SVZ) cell cultures contain mixed populations of immature cells, neurons, astrocytes, and progenitors in different stages of development. In the present work, we examined whether cell types of the SVZ could be functionally discriminated on the basis of intracellular free calcium level ([Ca2+]i) variations following KCl and histamine stimulation. For this purpose, [Ca2+]i were measured in SVZ cell cultures from neonatal P1-3 C57Bl/6 donor mice, in single cells, after stimulation with 100 μM histamine or 50 mM KCl. MAP-2-positive neurons and doublecortin-positive neuroblasts were distinguished on the basis of their selective ratio of response to KCl and/or histamine stimulation. Moreover, we could distinguish immature cells on the basis of the selective response to histamine via the histamine 1 receptor activation. Exposure of SVZ cultures to the pro-neurogenic stem cell factor (SCF) induced an increase in the number of cells responding to KCl and a decrease in the number of cells responding to histamine, consistent with neuronal differentiation. The selective response to KCl/histamine in single cell calcium imaging analysis offers a rapid and efficient way for the functional discrimination of neuronal differentiation in SVZ cell cultures, opening new perspectives for the search of potential pro-neurogenic factors.
机译:脑室下带(SVZ​​)细胞培养物包含处于不同发育阶段的未成熟细胞,神经元,星形胶质细胞和祖细胞的混合种群。在目前的工作中,我们检查了是否可以根据KCl和组胺刺激后细胞内游离钙水平([Ca2 +] i)的变化在功能上区分SVZ的细胞类型。为此,在用100μM组胺或50 mM KCl刺激后,在新生P1-3 C57Bl / 6供体小鼠的SVZ细胞培养物中的单个细胞中测量了[Ca2 +] i。 MAP-2阳性神经元和双皮质素阳性神经母细胞是根据它们对KCl和/或组胺刺激的选择性反应比率来区分的。此外,我们可以基于组胺1受体激活对组胺的选择性反应来区分未成熟细胞。 SVZ培养物暴露于前神经原性干细胞因子(SCF)导致对KCl的反应细胞数量增加,对组胺的反应细胞数量减少,这与神经元分化一致。在单细胞钙成像分析中对KCl /组胺的选择性反应为SVZ细胞培养物中神经元分化的功能判别提供了一种快速有效的方法,为寻找潜在的促神经原性因子开辟了新的前景。

著录项

  • 来源
    《Rejuvenation Research》 |2008年第1期|p.187-200|共14页
  • 作者单位

    Fabienne AgasseNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Liliana BernardinoNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Bruno SilvaNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Raquel FerreiraNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Sofia GradeNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.João O. MalvaNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Fabienne AgasseNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Liliana BernardinoNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Bruno SilvaNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Raquel FerreiraNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Sofia GradeNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.João O. MalvaNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Fabienne AgasseNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Liliana BernardinoNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Bruno SilvaNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Raquel FerreiraNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.Sofia GradeNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.João O. MalvaNeuroprotection and Neurogenesis in Brain Repair Group, Center for Neuroscience and Cell Biology, Institute of Biochemistry, Faculty of Medicine, University of Coimbra, Coimbra, Portugal.;

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  • 正文语种 eng
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  • 关键词

    Subventricular zone,potential pro-neurogenic factors;

    机译:脑室下区;潜在的前神经原性因子;

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