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首页> 外文期刊>Renewable energy >Microwave assisted pretreatment of eucalyptus sawdust enhances enzymatic saccharification and maximizes fermentable sugar yield
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Microwave assisted pretreatment of eucalyptus sawdust enhances enzymatic saccharification and maximizes fermentable sugar yield

机译：微波辅助对桉木屑的预处理可增强酶促糖化作用并最大程度提高可发酵糖的产量

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摘要

There is an urgent need of sustainable and efficient methods for the production of biofuels and chemicals from lignocellulosic feedstock. The purpose of this study was to develop a mild, cost-effective and environmentally benign pretreatment for woody lignocellulose to maximize sugar yield via enzymatic saccharification. Microwave irradiation (MW) of Eucalyptus regnans sawdust in water was investigated and compared directly against conventional liquid hot water (LHW) pretreatment. Following 30 min microwave irradiation at 180 degrees C, the sugar yield was 3.5 times higher using MW than LHW pretreatment under the same conditions. Complete release of C5 and C-6 sugars was achieved after the two-step method of MW pretreatment followed by enzymatic hydrolysis, compared with only 4% without pretreatment and 31% after LHW. Removal of 'lignin droplets' formed on the surface of the pretreated fibers via flowing hot water showed only minor improvement in the yield of enzymatic saccharification. Our results support the hypothesis that lignin prevents access of enzymes rather than inhibits their activity. MW accelerated depolymerization of hemicellulose, opening the structure more than LHW pretreatment. (C) 2018 Elsevier Ltd. All rights reserved.
机译：迫切需要可持续和有效的方法，以木质纤维素原料生产生物燃料和化学品。这项研究的目的是开发一种温和，经济，环保的木质木质纤维素预处理方法，以通过酶促糖化作用最大程度地提高糖产量。研究了桉树锯末在水中的微波辐射（MW），并直接与常规液态热水（LHW）预处理进行了比较。在180摄氏度下进行30分钟的微波辐射后，在相同条件下使用MW进行的糖收率是LHW预处理的3.5倍。 MW预处理两步法随后进行酶促水解后，C5和C-6糖完全释放，而未经预处理的只有4％，而LHW处理后只有31％。经由流动的热水去除在预处理的纤维表面上形成的“木质素小滴”显示出酶促糖化的收率仅有很小的提高。我们的结果支持以下假设：木质素可阻止酶的进入，而不是抑制酶的活性。 MW加速了半纤维素的解聚，比LHW预处理更开放了结构。 （C）2018 Elsevier Ltd.保留所有权利。

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