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首页> 外文期刊>Journal of natural fibers >Silk Degumming and Utilization of Silk Sericin by Hydrolysis Using Alkaline Protease from Beauveria Sp. (MTCC 5184): A Green Approach
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Silk Degumming and Utilization of Silk Sericin by Hydrolysis Using Alkaline Protease from Beauveria Sp. (MTCC 5184): A Green Approach

机译：球孢白僵菌碱性蛋白酶水解对蚕丝胶的脱胶和利用。 （MTCC 5184）：绿色方法

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Conventionally, degumming is carried out at 90 degrees C-110 degrees C temperature by boiling the raw silk with Marseilles soap and sodium bicarbonate which eventually requires a lot of water and energy. In this study, degumming of Chinese bivoltine raw silk fibres with alkaline protease produced by Beauveria sp. (MTCC 5184) is studied. Complete degumming was obtained in 45 min with 75 units of enzyme per gram of silk. Degumming was found to be optimal at 50 degrees C and pH 9.0. Scanning electron micrographs showed that the sericin deposits were removed and the obtained fibres were clean, separated, had smooth feel with shine as compared to untreated fibres. Sericin isolated from silk cocoon (by-product which goes waste) was hydrolyzed with the same alkaline protease obtained from Beauveria sp. to get small molecular weight peptides. These peptides can be utilized further for cosmetic, pharmaceutical, and various industrial applications.
机译：常规地，通过用马赛肥皂和碳酸氢钠煮沸生丝来在90℃至110℃的温度下进行脱胶，这最终需要大量的水和能量。在这项研究中，用白僵菌产生的碱性蛋白酶对中国双伏特原丝纤维进行脱胶。 （MTCC 5184）进行了研究。在45分钟内使用每克丝绸75个单位的酶获得完全脱胶。发现在50℃和pH 9.0下脱胶是最佳的。扫描电子显微镜照片显示，与未处理的纤维相比，丝胶蛋白沉积物被去除并且所获得的纤维清洁，分离，具有光滑的光泽感和光泽。从蚕茧分离得到的丝胶蛋白（变成废品的副产物）用从白僵菌得到的相同碱性蛋白酶水解。得到小分子量的肽。这些肽可进一步用于化妆品，药​​物和各种工业应用。

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