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首页> 外文期刊>Review of Agricultural Entomology >Analysis of Genetic Diversity and Phylogenetic Relationship of Kenaf Germplasm by SRAP
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Analysis of Genetic Diversity and Phylogenetic Relationship of Kenaf Germplasm by SRAP

机译：SRAP分析红麻种质的遗传多样性和系统发生关系。

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摘要

The genetic diversity and phylogenetic relationship were analyzed by sequence-related amplified polymorphism (SRAP) marker system on 84 varieties of kenaf fHibiscus cannabinus L.) germplasm collected from 26 countries and regions around the world. Twenty-six of total 225 SRAP primer combinations showed polymorphisms. Analysis of 84 kenaf varieties by using the 26polymorphic primer combinations had identified a total of 329 reproducible bands. Among them, 248 bands (75.4%) were polymorphic. The number of polymorphic fragments produced by an individual primer combination ranged from 5 to 15, with an average of 9.54 bands. DNA fragment size ranged from 300 bp to 1500 bp. Cluster analysis of the SRAP fingerprints clearly separated the 84 varieties into three distinct groups: cultivar, wild, and intermediate types. The cultivars were further delineated into four subgroups. High genetic similarity was found among varieties from the same geographic region, resulting from the fact that kenaf is a plant dominated by cross-pollination. This research indicated that SRAP molecular marker could be used to perform kenaf varieties identification, genetic diversity and phylogenetic relationship assessment, and germplasm evaluation.
机译：利用序列相关扩增多态性（SRAP）标记系统分析了来自世界26个国家和地区的84个洋麻木槿种质的遗传多样性和系统发育关系。总共225个SRAP引物组合中有26个显示多态性。通过使用26种多态性引物组合对84个洋麻品种进行分析，共鉴定出329条可重复条带。其中，248条带（75.4％）是多态的。单个引物组合产生的多态性片段数范围为5至15，平均有9.54条带。 DNA片段大小在300bp至1500bp之间。 SRAP指纹的聚类分析清楚地将84个品种分为三个不同的组：品种，野生和中间类型。品种进一步划分为四个亚组。在同一地理区域的变种中发现高度遗传相似性，这是由于洋麻是一种以异花授粉为主的植物。研究表明SRAP分子标记可用于洋麻品种的鉴定，遗传多样性和系统关系的评估以及种质评价。

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  • 来源
    《Review of Agricultural Entomology》 |2011年第2期|p.99-110|共12页
  • 作者

    JIANMIN QI; JIANTANG XU; AIQING LI; XIAOFEI WANG; GUANGQING ZHANG; JIANGUANG SU; AIMIN LIU;

  • 作者单位

    Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops (Fujian Agriculture and Forestry University), Fuzhou, Fujian, People's Republic of China;

    Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops (Fujian Agriculture and Forestry University), Fuzhou, Fujian, People's Republic of China;

    Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops (Fujian Agriculture and Forestry University), Fuzhou, Fujian, People's Republic of China,Anbui Seed Management Station, Hefei, People's Republic of China,Hunan Agriculture University, Wuhan, People's Republic of China;

    Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops (Fujian Agriculture and Forestry University), Fuzhou, Fujian, People's Republic of China;

    Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops (Fujian Agriculture and Forestry University), Fuzhou, Fujian, People's Republic of China;

    Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops (Fujian Agriculture and Forestry University), Fuzhou, Fujian, People's Republic of China,Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan, People's Republic of China;

    University of Minnesota, St. Paul, MN, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    kenaf; srap; genetic diversity; dna molecular marker;

    机译：红麻废话遗传多样性;和分子标记;

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