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首页> 外文期刊>Journal of natural fibers >Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and Phosphogypsum
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Occurrence of Arbuscular Mycorrhizal Fungi in Hemp (Cannabis sativa) Plants and Soil Fertilized with Sewage Sludge and Phosphogypsum

机译：大麻（Cannabis Sativa）植物和用污水污泥和磷酸缺口施肥的丛枝菌根真菌的发生

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The colonization of soil and roots by arbuscular mycorrhizal fungi and the formation of spores under the influence of fertilization with phosphogypsum and sewage sludge were examined. Identification of arbuscular mycorrhizal fungi was carried out in soil and in roots of outdoor experimental hemp plantation. Assessment of the colonization was carried out by molecular and microscopic methods. The material for the isolation of DNA consisted of soil samples taken from mycorrhizal soil and hemp roots of cv. Bialobrzeskie, cv. Tygra, and cv. Beniko. In nested PCR reactions part of the small subunit (SSU) region, the region of the internal transcribed spacer (ITS) and part of large subunit (LSU) rDNA region were amplified. As a result of the cloning and sequencing of the DNA, it was found that the greatest similarity of the obtained sequences present in the samples of roots and soil was to the following species: Diversispora sp., Funneliformis sp., Glomus sp., Funneliformis mosseae, Glomus caledonium, Funneliformis geosporum, Glomus occultum. The colonization by the fungi was estimated and found to differ depending on the hemp cultivar and doses of fertilizers. Cultivar Tygra showed no negative impact on biomass production by intensive mycorrhization, proved its high tolerance to phosphogypsum and sewage sludge pollution, and responded positively regarding biomass production.
机译：采用丛枝菌根真菌的土壤和根部定植，并在磷酸缺口施肥和污水污泥的影响下形成孢子的形成。常规菌根真菌的鉴定是在土壤中和室外实验大麻种植植物的根系中进行。通过分子和微观方法进行殖民化评估。用于分离DNA的材料由来自菌根土壤和CV的大麻根的土壤样品组成。 bialobrzeskie，cv。 Tygra和CV。班戈。在嵌套的PCR反应部分小亚基（SSU）区域，扩增内部转录间隔物（其）和一部分的大亚基（LSU）RDNA区域的区域。由于DNA的克隆和测序，发现所获得的序列的最大相似性在根和土壤样品中存在于以下物种：Diversispora Sp。，Filneliformis sp。，Glomus sp。，filneliformis Mossea，Glomus Caledonium，Filneliformis Geosporum，Glomus Occultum。估计真菌的定植，并发现取决于大麻栽培品种和剂量的肥料不同。品种TYGRA对深性腐蚀性显示出对生物量产生的负面影响，证明了其对磷酸缺口和污水污泥污染的高耐受性，并对生物质生产进行了肯定的作用。

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