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A bright and long-pulse illumination for ultrahigh-speed microscopy of living specimens

机译：高亮长脉冲照明，用于活体标本的超高速显微镜检查

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Ultrahigh-speed microscopy of living specimens requires ultrabright illumination. Moreover, the duration of illumination should be sufficiently long, on the order of at least several tens of milliseconds, in order to investigate the dynamic state of living specimens. However, specimens are exposed to a high risk of damage by the intense illumination. The brightness and pulse duration of illumination have to be continuously controlled for use in the ultrahigh-speed microscopy of living specimens. Commercial or laboratory-made illumination systems do not satisfy the abovementioned requirements. In this paper, the development of a bright and long-pulse illumination system for ultrahigh-speed microscopy of living specimens is presented. A xenon flashlamp with an arc length of 1.5 mm has been used as the light source. The electrical power supply consists of a voltage-regulated circuit, a capacitor bank, and a control circuit including an insulated-gate bipolar transistor as a gating device, which provides a large rectangular current pulse with the duration in the range to the order of several tens of milliseconds. The brightness, pulse duration, and repetition rate can be easily and continuously controlled. The illumination developed in the present study is installed in an inverted fluorescence microscope equipped with a high-speed camera in order to evaluate the performance as an illumination source. A fluorescent image of the living spermatozoa of a mouse obtained at a frame rate of 8 kHz shows good contrast. Such an image cannot be obtained using a commercial illumination system. © 2010 American Institute of Physics Article Outline INTRODUCTION Condition setting Observation LIGHT SOURCES ELECTRICAL CIRCUIT AND FLASH LIGHT MEASUREMENT HIGH-SPEED IMAGING OF LIVING SPECIMENS CONCLUSIONS
机译：活体标本的超高速显微镜检查需要超亮照明。此外，照明的时间应足够长，至少几十毫秒，以便研究活体标本的动态状态。但是，样品在强光照射下很容易遭受损坏。为了在活样本的超高速显微镜中使用，必须连续控制照明的亮度和脉冲持续时间。商业或实验室制造的照明系统不满足上述要求。在本文中，提出了用于活体标本的超高速显微镜的明亮长脉冲照明系统的开发。弧长为1.5 mm的氙气闪光灯已用作光源。电源由调压电路，电容器组和控制电路组成，控制电路包括一个绝缘栅双极晶体管作为门控设备，该电路提供一个大的矩形电流脉冲，其持续时间在几倍的范围内数十毫秒。亮度，脉冲持续时间和重复频率可以轻松连续地控制。本研究开发的照明装置安装在配备有高速相机的倒置荧光显微镜中，以评估其作为照明源的性能。以8 kHz的帧频获得的小鼠活精子的荧光图像显示出良好的对比度。使用商业照明系统无法获得这种图像。 ©2010美国物理研究所文章大纲简介条件设置观察光源电路和闪光灯测量生活标本的高速成像结论

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