Pulse fluorometry using simultaneous acquisition of fluorescence and excitation

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Pulse fluorometry using simultaneous acquisition of fluorescence and excitation

机译：使用同步采集荧光和激发的脉冲荧光法

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We report a new method of measuring fluorescence lifetimes which uses the single‐photon technique and two detection channels with matched impulse response for simultaneous acquisition of fluorescence and excitation (SAFE). This differential arrangement is shown to correct automatically for variations in the optical pulse profile during the measurement, thus eliminating a common source of error. It can be used to improve precision and sensitivity with any pulsed light source such as a flashlamp, laser, or synchrotron. A routing system separates photomultiplier coincidences from the dual detection channels into different memory segments of a multichannel analyzer (MCA) using a single time‐to‐amplitude converter (TAC). Comprehensive data are presented on tuning the single‐photon response of the Philips range of XP2020Q photomultipliers. Results obtained using a coaxial flashlamp to excite a dilute solution of PPO in ethanol give a lifetime of 1.63±0.02 ns in good agreement with that obtained using conventional fluorometry. The method is also useful in the study of dual emissions such as in monomer–excimer systems and in the measurement of time‐resolved emission anisotropy.

机译：我们报告了一种测量荧光寿命的新方法，该方法使用单光子技术和两个具有匹配脉冲响应的检测通道来同时捕获荧光和激发光（SAFE）。示出了这种差分布置以在测量期间自动校正光脉冲轮廓的变化，从而消除了常见的误差源。它可用于与任何脉冲光源（如闪光灯，激光或同步加速器）一起提高精度和灵敏度。路由系统使用单个时间振幅转换器（TAC）将光电倍增管的重合从双检测通道分离到多通道分析仪（MCA）的不同存储段中。提供了有关调整Philips系列XP2020Q光电倍增管的单光子响应的全面数据。使用同轴闪光灯激发PPO在乙醇中的稀溶液获得的结果与传统荧光法获得的寿命一致，寿命为1.63±0.02 ns。该方法还可用于研究双发射，例如单体准分子系统，以及测量时间分辨的发射各向异性。

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《Review of Scientific Instruments》 |1984年第8期|P.1255-1264|共10页
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1. Autoregressive-Model-Based Fluorescence-Lifetime Measurements by Phase-Modulation Fluorometry Using a Pulsed-Excitation Light Source and a High-Gain Photomultiplier Tube [J] . Applied Spectroscopy . 2009,第11期

机译：通过使用脉冲激发光源和高增益光电倍增管的相位调制荧光法，基于自回归模型的荧光寿命测量
2. Autoregressive-Model-Based Fluorescence-Lifetime Measurements by Phase-Modulation Fluorometry Using a Pulsed-Excitation Light Source and a High-Gain Photomultiplier Tube [J] . TETSUO IWATA, RITSUKI ITO, YASUHIRO MIZUTANI, Applied Spectroscopy: Society for Applied Spectroscopy . 2009,第11期

机译：通过使用脉冲激发光源和高增益光电倍增管的相位调制荧光法，基于自回归模型的荧光寿命测量
3. Pulsed excitation source multiplexed fluorometry for the simultaneous measurement of multiple analytes. Continuous measurement of atmospheric hydrogen peroxide and methyl hydroperoxide [J] . Li JZ., Dasgupta PK., Tarver GA. Analytical chemistry . 2003,第5期

机译：脉冲激发源多路荧光法可同时测量多种分析物。连续测量大气中的过氧化氢和氢过氧化甲基
4. Polarized fluorescence in NADH in water/methanol solutions upon excitation with femtosecond laser pulses [C] . I.A. Gorbunova, M.E. Sasin, N.O. Bezverkhnii, International Conference Laser Optics . 2020

机译：用飞秒激光脉冲激发，在水/甲醇溶液中的偏振荧光在水/甲醇溶液中
5. Structural characteristics of metal oxide thin films grown by pulsed laser deposition [and] fluorescence excitation spectroscopy of amino radical, fluoromethylene, and chlorocarbene under jet-cooled condition and measurement of fluorescence lifetime. [D] . Fan, Haiyan. 2004

机译：在脉冲冷却条件下通过脉冲激光沉积[和]自由基，氟代亚甲基和氯卡宾的荧光激发光谱和原子荧光测量方法生长的金属氧化物薄膜的结构特征，以及荧光寿命的测量。
6. Low Peak Power Multiband Spokes Pulses for ... formula ... Inhomogeneity-Compensated Simultaneous Multislice Excitation in High Field MRI [O] . Anuj Sharma, Roland Bammer, V. Andrew Stenger, -1

机译：低峰峰值功率多频带辐照脉冲用于高场MRI中不均匀性补偿的同时多层激励
7. Light Quenching of Fluorescence Using Time-Delayed Laser Pulses As Observed by Frequency-Domain Fluorometry [O] . Ignacy Gryczynski, Jozef Kusba, Joseph R. Lakowicz 1994

机译：使用频域荧光测定法观察的时滞激光脉冲光淬火荧光
8. Fluorescence detection of single molecules using pulsed near-field optical excitation and time correlated photon counting [R] . Ambrose, W. P., Goodwin, P. M., Martin, J. C., 1994

机译：使用脉冲近场光学激发和时间相关光子计数的单分子荧光检测

Pulse fluorometry using simultaneous acquisition of fluorescence and excitation

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