High pressure stopped‐flow apparatus for the rapid mixing and subsequent study of two fluids under high hydrostatic pressures

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High pressure stopped‐flow apparatus for the rapid mixing and subsequent study of two fluids under high hydrostatic pressures

机译：高压停流装置，用于在高静水压力下快速混合并随后研究两种流体

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A stopped‐flow apparatus is described for the rapid mixing and subsequent study of two dissimilar fluids under pressures up to 1200 bar. The device consists of two identical pressure chambers which contain the two fluids, a third pressure chamber which contains gas to maintain the pressure in the system, an optical port for photometric observation, and various connections. The device has been used to measure reaction times on the order of a hundred milliseconds to tens of seconds, using a maximum of 2 ml of each reagent per experimental determination. The dead time is found to be 5–25 ms with minium average flow velocities of 2.0 m/s. The construction and operation of the device are described and examples of water transport data in red blood cells and the bromophenolblue indicated chemical reaction of NaHCO3 and HCl under pressure are presented.

机译：描述了一种停流装置，用于在压力高达1200 bar的情况下快速混合并随后研究两种不同的流体。该设备由包含两个流体的两个相同的压力室，包含用于维持系统压力的气体的第三个压力室，用于光度法观察的光学端口以及各种连接方式组成。该设备已用于测量反应时间，范围从100毫秒到数十秒不等，每个实验测定最多使用2 ml每种试剂。死区时间为5–25 ms，最小平均流速为2.0 m / s。描述了该装置的结构和操作，并举例说明了红细胞中水传输数据的例子，并介绍了溴酚蓝指示的NaHCO3和HCl在压力下的化学反应。

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《Review of Scientific Instruments》 |1980年第8期|P.1042-1045|共4页
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High pressure stopped‐flow apparatus for the rapid mixing and subsequent study of two fluids under high hydrostatic pressures

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