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首页> 外文期刊>Review of Scientific Instruments >Design, development, and performance of a fiber optics refractometer: Application to HPLC
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Design, development, and performance of a fiber optics refractometer: Application to HPLC

机译：光纤折光仪的设计，开发和性能：在HPLC中的应用

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摘要

A fiber optics refractometer has been developed based on a simple low‐volume cell (5–7 μl) which confines the liquid to be measured around a waveguide whose multiple reflections can enhance the contrast at the critical angle. The electromechanical and electrooptical design allows the fiber optics refractometer to measure absolute refractive index with manual or automatic readout, and differential operation with automatic tracking that permits its use as a universal detector for gradient elution chromatography. Present sensitivity is approximately 1×10-5 refractive index units in the static mode and 7×10-5 refractive index units in the scanning beam mode. These sensitivities can probably be improved by a factor of 5 by appropriate system changes.
机译：光纤折光仪是基于简单的小体积样品池（5-7μl）开发的，该样品池将待测液体限制在波导周围，其多次反射可以提高临界角处的对比度。机电和电光设计允许光纤折光仪通过手动或自动读数来测量绝对折射率，并通过自动跟踪进行差分操作，从而使其可用作梯度洗脱色谱法的通用检测器。当前灵敏度在静态模式下约为1×10-5折射率单位，在扫描光束模式下约为7×10-5折射率单位。通过适当的系统更改，这些灵敏度可能会提高5倍。

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