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首页> 外文期刊>Russian journal of marine biology >Cell Differentiation during the Larval Development of the Ophiuroid Amphipholis kochii Liitken, 1872 (Echinodermata: Ophiuroidea)
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Cell Differentiation during the Larval Development of the Ophiuroid Amphipholis kochii Liitken, 1872 (Echinodermata: Ophiuroidea)

机译:蚜虫幼虫发育过程中的细胞分化1872(Echinodermata:Ophiuroidea)

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摘要

The differentiation of the ectodermal, entodermal, and mesodermal cell lines in developing plutei of the ophiumd Amphipholis kochii was examined using electron microscopy and the immunochemical staining technique. The ectodermal cells form the pseudostratified epithelium of the ciliary band, the flattened epithelium of the body wall, and the esophageal epithelium. The epithelium of the ciliary band consists of ciliated and mucous cells; at its base is an axonal tract formed of the processes of neurons. The serotoninergic neurons form two lateral ganglia located along the paraoral ciliary band and the posterolateral arms' ciliary band. The prominent features of the neurons are large size, the presence of a cilium, an electron-light cytoplasm filled with microvesicles with neurotransmitters, and a large nucleus with a predominant euchromatin. The ectoderm cells (except mucous cells) are characterized by the presence of a cilium surrounded by a collar of microvilli and a thin layer of apical extracellular matrix. The entodermal cells form the digestive tract epithelium and differentiate into four cell types: type I and II cells probably function in the nutrient uptake and assimilation; type III cells perhaps secrete digestive enzymes; and myoepithelial cells that constitute the cardiac and pyloric sphincters and the anus. Sclerenchymatous cells, which are the descendants of the primary mesenchyme, form a syncytium around the developing spicules. The biominerah'zation process is intrasyn-cytial, the ophioplutei spicules retain the cytoplasmic covering throughout the period of larval development. The secondary mesenchyme gives rise to smooth muscle cells and amebocytes. Muscle cells compose the cir-cumesophageal musculature, the cell processes of each "muscle band" seem to fuse together. At the base of the preoral band are two symmetrically located groups of muscles, viz., the anterior dilators. Amebocytes function in excretion either near the epidermis or are able to penetrate through the epidermis and excrete wastes into the external environment. The mesoderm formed by the enterocoely gives rise to three pairs of coeloms; their cells remain unspecialized during the entire period of larval development. Results of this study are compared with the micro- and neuroanatomy of the larvae of other echinoderms.
机译:使用电子显微镜和免疫化学染色技术,检查了发育中的ophiumd Amphipholis kochii的plutei中的外胚层,内胚层和中胚层细胞系的分化。外胚层细胞形成睫状带的假复层上皮,体壁的扁平上皮和食道上皮。睫状带的上皮由纤毛和粘液细胞组成。在其基部是由神经元过程形成的轴突束。 5-羟色胺能神经元形成两个旁神经节,它们位于口旁睫状带和后外侧臂的睫状带。神经元的显着特征是大尺寸,纤毛的存在,充满神经递质的微囊泡填充的电子光细胞质,以及具有主要常染色质的大核。外胚层细胞(粘液细胞除外)的特征是存在纤毛周围被纤毛和顶端细胞外基质薄层包围的纤毛。表皮细胞形成消化道上皮并分化为四种细胞类型:I型和II型细胞可能在营养吸收和吸收中起作用; III型细胞可能分泌消化酶;以及构成心脏和幽门括约肌和肛门的肌上皮细胞。硬化性细胞是原代间质的后代,在发育中的针状体周围形成合胞体。生物矿化过程是突触内的,在幼体发育的整个过程中,ophioplutei针状体保留了细胞质覆盖。次生间充质产生平滑肌细胞和变形细胞。肌肉细胞组成了cir-cumesophageal肌肉组织,每个“肌肉带”的细胞过程似乎融合在一起。在前带的基部是两组对称分布的肌肉,即前扩张器。变形细胞在表皮附近排泄中起作用,或者能够穿透表皮并将废物排泄到外部环境中。由肠形成的中胚层产生三对腔肠;在幼虫发育的整个过程中,它们的细胞仍未专一化。将该研究结果与其他棘皮动物幼虫的显微和神经解剖学进行比较。

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