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首页> 外文期刊>Russian journal of marine biology >Induction of Polyploidy by Colchicine on the Green Algae Dunaliella salina
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Induction of Polyploidy by Colchicine on the Green Algae Dunaliella salina

机译：秋水仙碱对绿藻杜氏盐藻的多倍体诱导

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摘要

In this study, induction of polyploidy by colchicine was investigated in Dunaliella salina. To induce polyploidy in the cells, different concentrations of colchicine (0.05, 0.1, 0.5 and 1%) were used in three periods of times 12, 24, and 36 h. Measurement of DNA content was done by Nano drop method. All concentrations of colchicine caused an increase in DNA content. Results showed that the highest value of DNA obtained related to algal cells were exposed to 1.0 and 0.5% colchicine for 36 h (0.213 g/L and 0.274 g/L, respectively), with respect to DNA content in control sample, these treatments caused induction of polyploidy in algal cells. Survival percentage of cells at all concentrations of colchicine significantly decreased. Based on obtained results from survival rate and DNA content, two concentrations of 0.1 and 0.5% colchicine were selected for other analysis. Colchicine treatments (0.1 and 0.5%) caused an increase in doubling time and specific growth rate declined in the treated algae. Pictures obtained by fluorescence microscopy and image analysis revealed a significant enhancement of the cell and nuclear size of the microalgae. Results of flow cytometry analysis indicated that percentage of polyploid cells in cultures treated by 0.1% colchicine was 58.26% and by 0.5% colchicine was 74.19%.
机译：在这项研究中，在杜氏盐藻中研究了秋水仙碱诱导多倍体的能力。为了在细胞中诱导多倍性，在12、24和36小时的三个时间段中使用了不同浓度的秋水仙碱（0.05％，0.1％，0.5％和1％）。 DNA含量的测定通过纳米滴法进行。所有浓度的秋水仙碱引起DNA含量的增加。结果表明，相对于对照样品中的DNA含量，与藻类细胞相关的DNA最高值暴露于1.0％和0.5％秋水仙碱中36小时（分别为0.213 g / L和0.274 g / L），这些处理导致在藻类细胞中诱导多倍性。在所有秋水仙碱浓度下，细胞的存活百分比均显着降低。根据生存率和DNA含量获得的结果，选择0.1和0.5％秋水仙碱的两种浓度进行其他分析。秋水仙碱处理（0.1和0.5％）导致处理时间增加了一倍，并且比生长速率下降。通过荧光显微镜和图像分析获得的图片显示微藻的细胞和核大小显着增强。流式细胞仪分析的结果表明，用0.1％秋水仙碱处理的培养物中多倍体细胞的百分比为58.26％，而用0.5％秋水仙碱处理的为74.19％。

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