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首页> 外文期刊>Russian journal of marine biology >Fungi in Bottom Sediments of the Chukchi Sea
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Fungi in Bottom Sediments of the Chukchi Sea

机译：Chukchi海底沉积物的真菌

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摘要

We present the first study on the quantity and diversity of the mycobiota in bottom sediments of the Chukchi Sea. During implementation of the RUSALCA-2012 program, 22 samples of bottom sediments from depths of 44-110 m were collected in late August-early September 2012. Fungi were isolated on agarized media (salinity 35 psu, temperature 6 degrees C). Species identification was performed by morphological-cultural and molecular-genetic methods based on the nucleotide sequences of ITS1-ITS2 rDNA. A total of 128 colonies of mycelial fungi were isolated; 0 to 22 propagules were obtained from each 1 cm(3) sample. The overall diversity was represented by 48 morphotypes belonging to 32 genera in 15 orders of ascomycetes and basidiomycetes. Ascomycetes predominated both in number and diversity. A number of species were found in marine ecosystems for the first time. The taxonomic position of nonsporulating isolates (13% of the total) was established using molecular techniques. Among them, high diversity was observed in the orders Pleosporales and Helotiales (Ascomycota).
机译：我们介绍了丘脑海底底部沉积物中霉菌菌的数量和多样性研究。在实施Rusalca-2012计划期间，在2012年8月初期的44-110米中收集了22个底部沉积物的样本。真菌被隔离在激介质（盐度35psu，温度6摄氏度）上分离出来。基于ITS1-ITS2 rDNA的核苷酸序列，通过形态学 - 培养和分子遗传方法进行物种鉴定。共分离了128个菌丝菌丝菌丝菌群;从每一个1cm（3）个样品获得0至22个拍摄。整体多样性由48种不同的蛋白质在15个倒数症状和碱性细胞中属于32个属。 ascomycetes在数量和多样性中占据主义。第一次在海洋生态系统中发现了许多物种。采用分子技术建立了戒烟分离株的分类物理位置（总量的13％）。其中，在订单葡萄球菌和Helotiales（Ascomycota）中观察到高多样性。

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