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首页> 外文期刊>Russian journal of marine biology >Structure Analysis of Mitochondrial DNA Control Region Sequences and its Applications for the Study of Population Genetic Diversity of Acanthogobius ommaturus
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Structure Analysis of Mitochondrial DNA Control Region Sequences and its Applications for the Study of Population Genetic Diversity of Acanthogobius ommaturus

机译：线粒体DNA对照区序列序列的结构分析及acanthobius omomaturus群体遗传多样性研究

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摘要

Acanthogobius ommaturus, which belongs to the family Gobiidae, is a euryhaline, demersal fish widely distributed in coastal areas, harbors, and estuaries of China, the Korean Peninsula, and Japan. A genetic analysis based on identification of the structure of mitochondrial DNA control region was carried out in the present study. As a result, the conservative structure, including termination sequence region, central conservative region CSB-E and CSB-D, as well as conservative sequence region CSB-1, CSB-2, and CSB-3, was determined. A total of 32 polymorphic sites were identified and 33 haplotypes were defined, of which only 9 were shared haplotypes. A high level of haplotype diversity (h= 0.923 +/- 0.018) and low nucleotide diversity (pi = 0.003 +/- 0.002) were detected. In general, the genetic differentiation among ten populations was at a medium level. There was a distinct single peak in the nucleotide mismatch distribution, which confirms thatA. ommaturuspopulations have experienced recent expansion. In this study, the control region structure ofA. ommaturuswas similar to that of most marine fishes; however, the tandem repeat sequence and the CSB-F sequence were not detected. In addition, most of the genetic variation in the control region occurred in the high-variation region.
机译：acanthogobius ommaturus属于gobiidae，是一个euryhaline，沿海地区，港口和河口和日本的沿海地区，港口和河口广泛分布。在本研究中进行了基于线粒体DNA对照区结构鉴定的遗传分析。结果，确定了保守结构，包括终止序列区域，中央保守区CSB-E和CSB-D以及保守序列区域CSB-1，CSB-2和CSB-3。鉴定了总共32个多晶型位点，定义了33个单倍型，其中只有9个被共用单倍型。检测到高水平的单倍型多样性（H = 0.923 +/- 0.018）和低核苷酸多样性（PI = 0.003 +/- 0.002）。通常，十种种群的遗传分化在中等水平。核苷酸不匹配分布中存在明显的单峰，这证实了Qua。 omomaturuspopopations经历了最近的扩张。在本研究中，控制区域结构OF。 ommaturuswas类似于大多数海洋鱼类;但是，未检测到串联重复序列和CSB-F序列。另外，控制区域的大多数遗传变化发生在高变形区域中。

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