Proteinases with Gelatinase Activity and their Role in Ambulacrum Regeneration in Holothurians Eupentacta fraudatrix (D'yakonov and Baranova, 1958) and Cucumariajaponica (Semper, 1868) (Echinodermata: Holothuroidea)

Shulga A. P.; Lamash N. E.

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Proteinases with Gelatinase Activity and their Role in Ambulacrum Regeneration in Holothurians Eupentacta fraudatrix (D'yakonov and Baranova, 1958) and Cucumariajaponica (Semper, 1868) (Echinodermata: Holothuroidea)

机译：具有明胶酶活性的蛋白酶及其在Holothurans Eupentacta Fraudatrix（D'Yakonov和Baranova，1958）和Cucumariajaponica（Semper，1868）（Echinodermata：Holothuroidea）中的作用

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The spectrum of proteinases and the dynamics of proteinase activity in the ambulacrum structures were studied by quantitative gel zymography in the holothurians Eupentacta fraudatrix and Cucumaria japonica under normal conditions and after transverse cutting of the ambulacrum. Proteinases with gelatinolytic activity were detected only in E. fraudatrix; the species had two pools of proteinases. The high-molecular-weight pool was represented by only one (132 kDa) or two (132 and more than 250 kDa) proteins; the low-molecular-weight pool included two proteins with molecular weights of 53 and 47 kDa. In their ability to lyse gelatin, but not casein, and in their sensitivity to inhibitors of different classes of enzymes, gelatinases of E. fraudatrix are similar to vertebrate matrix metalloproteinases. It was shown that the activity of proteinases increased after injury to the animal and that GM6001, a selective inhibitor of metalloproteinases, blocks the proteinase activity. It was suggested that proteinases with gelatinolytic activity are a key factor of regeneration in holothurians.

机译：在正常条件下，通过定量凝胶酶测量，在正常条件下，在正常条件下，通过定量凝胶酶测定，在正常条件下以及横向切割后的蛋白质凝胶酶谱和蛋白质痤疮杂耍的蛋白酶活性蛋白酶活性的谱和动力学。仅在E. Fraudatrix中检测具有凝胶溶解活性的蛋白酶;物种有两池蛋白酶。高分子量池仅由一种（132kDa）或两（132 kda）（132和超过250kDa）蛋白表示;低分子量池包括两种分子量为53和47kDa的蛋白质。在它们能够粘合明胶，但不是酪蛋白的能力，以及它们对不同类别酶的抑制剂的敏感性，E.Fraudatrix的明胶酶类似于脊椎动物基质金属蛋白酶。结果表明，蛋白酶的活性在动物损伤后增加，并且该GM6001是金属蛋白酶的选择性抑制剂，阻断蛋白酶活性。建议具有凝胶溶解活性的蛋白酶是妓女再生的关键因素。

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《Russian journal of marine biology》 |2020年第6期|461-471|共11页
作者
Shulga A. P.; Lamash N. E.;
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Russian Acad Sci Far Eastern Branch Zhirmunsky Natl Sci Ctr Marine Biol Vladivostok 690041 Russia;

Russian Acad Sci Far Eastern Branch Zhirmunsky Natl Sci Ctr Marine Biol Vladivostok 690041 Russia|Russian Acad Sci Papanin Inst Biol Inland Waters Borok 152742 Russia;

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正文语种 eng
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holothurians; Eupentacta fraudatrix; Cucumaria japonica; ambulacrum; gelatinase; matrix metalloproteinases; regeneration;

机译：HOLOTHURIAN;EUPENTACTA FRAUDATRIX;Cucumaria japonica;ambulacrum;明胶酶;基质金属蛋白酶;再生;

Proteinases with Gelatinase Activity and their Role in Ambulacrum Regeneration in Holothurians Eupentacta fraudatrix (D'yakonov and Baranova, 1958) and Cucumariajaponica (Semper, 1868) (Echinodermata: Holothuroidea)

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