High-resolution backscatter electron detection of cell surface molecules on human platelets using the double-layer coating method and cryo field emission scanning electron microscopy

Stanley L.Erlandsen; AnneGreet Bittermann; Marlys Krumweide

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High-resolution backscatter electron detection of cell surface molecules on human platelets using the double-layer coating method and cryo field emission scanning electron microscopy

机译：双层包被方法和冷场发射扫描电子显微镜对人体血小板上细胞表面分子的高分辨率背散射电子检测

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摘要

A model system utilizing cryo scanning electron microscopy (SEM) for the detection of putative cell adhesion molecule(s) on the surface of human platelets is described. Plunge freezing was used for cryoimmobilization of unacti- Vated and activated platelets after prefixation. Extracellular Ice was removed by sublimation to expose the surface of the Platelet membrane. Cryosamples were coated by the double- Layer method, in which unidirectional shadowing is per- Formed at an angle of 45° with 2 nm of platinum by thermal Evaporation, followed by evaporation of 5 nm of carbon at an Angle of 90° for stabilization of the platinum film.

机译：描述了利用冷冻扫描电子显微镜（SEM）检测人血小板表面上假定的细胞粘附分子的模型系统。加前缀后，将冷冻冷冻用于未活化和活化的血小板的冷冻固定。通过升华除去细胞外冰以暴露血小板膜的表面。通过双层方法涂覆低温样品，其中通过热蒸发在45°角与2 nm铂进行单向遮蔽，然后在90°角蒸发5 nm的碳以稳定碳。铂膜。

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《Scanning》 |1997年第5期|p.356-360|共5页
作者
Stanley L.Erlandsen; AnneGreet Bittermann; Marlys Krumweide;
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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类光学仪器;
关键词
cryo scanning electron microscopy; double-layer coating; cell surface molecule;

机译：冷冻扫描电子显微镜双层涂层细胞表面分子;

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1. Double-Layer coating for field-emission Cryo-Scanning electron microscopy-Present state and applications [J] . P.Walther, M.Muller Scanning . 1997,第5期

机译：用于场发射低温扫描电子显微镜的双层涂层-现状和应用
2. Double-layer coating for field-emission cryo-scanning electron microscopy—present state and applications [J] . P. Walther, M. MÜller Scanning . 1997,第5期

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3. BACKSCATTERED ELECTRON IMAGING OF THE UNDERSURFACE OF RESIN-EMBEDDED CELLS BY FIELD-EMISSION SCANNING ELECTRON MICROSCOPY [J] . Richards RG., Apgwynn I. Journal of Microscopy . 1995,第Pta1期

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机译：场发射扫描电子低温显微镜对有机物和天然有机矿物质关系的研究的贡献。在土壤微生物结壳中的应用有机物和有机矿物质缔合的场发射低温扫描电子显微镜。在微生物土壤结皮中的应用

High-resolution backscatter electron detection of cell surface molecules on human platelets using the double-layer coating method and cryo field emission scanning electron microscopy

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