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Effect of Surface Conjugation Chemistry on the Sensitivity of Microcantilever Sensors

机译:表面共轭化学对微悬臂梁传感器灵敏度的影响

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摘要

Microcantilever sensors are an offshoot of atomic force microscopy and are useful tools for effectively detecting a target biomolecule. The recognition of the target molecule on the biosensor is based on the physical bending of the microcantilever, which is driven by a specific molecular interaction between the target molecule and the sensor surface. In this study, to enhance the sensitivity of the microcantilever sensor, the sensor surface was modified through a surface conjugation method using self-assembled monolayers (SAMs) and heterobifunctional cross-linkers. After the surface modification of the microcantilever sensor, the sensitivity for L-cysteine was recorded. The detection of L-cysteine was influenced by the active site and the molecular size of the cross-linked compound attached onto the surface of the microcantilever.
机译:微悬臂梁传感器是原子力显微镜的分支,是有效检测目标生物分子的有用工具。生物传感器上目标分子的识别基于微悬臂梁的物理弯曲,这是由目标分子与传感器表面之间的特定分子相互作用驱动的。在这项研究中,为提高微悬臂梁传感器的灵敏度,通过使用自组装单分子层(SAMs)和异双功能交联剂的表面偶联方法对传感器表面进行了修饰。对微悬臂梁传感器进行表面修饰后,记录了对L-半胱氨酸的敏感性。 L-半胱氨酸的检测受活性位点和附着在微悬臂梁表面的交联化合物分子大小的影响。

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