...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Science of advanced materials >In Vitro Characterization of Thermal Behaviour and Bone Marrow Stromal Cell Attachment on Chitosan/Polycaprolactone (CS/PCL) Nanofibrous Scaffolds
【24h】

In Vitro Characterization of Thermal Behaviour and Bone Marrow Stromal Cell Attachment on Chitosan/Polycaprolactone (CS/PCL) Nanofibrous Scaffolds

机译：壳聚糖/聚己内酯（CS / PCL）纳米纤维支架的热行为和骨髓基质细胞附着的体外表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Forming novel 3D fibrous scaffolds by mixing natural and synthetic polymers is very interesting and promising for many biomedical applications. Hybrid scaffolds made from fibers of chitosan and polycaprolactone are highly valuable for tissue engineering and regenerative medicine applications due to combining the benefits of both polymers to improve the mechanical, physical and biological properties needed for biomedical fields. To prepare the electrospinning solutions of PCL and CS mixture, a 10: 3 wt% was used and electrospun with an acceleration voltage of 20 kV after filtering with a 0.1 mm PTFE membrane. Morphological observation indicated that the fibers are of uniform diameter (200-500 nm). Differential scanning calorimetry and thermogravimetric analyzer were used to characterize the thermal behaviour of CS/PCL nanofibers. Further, the scaffolds were tested with human telomerized (hTERT) human bone marrow (hBM)-mesenchymal (MSC). The cells were seeded at a density of 0.05x10(6) cells on each CS/PCL nanofibrous scaffold and cultured on the scaffolds for 6 days. The bone marrow stromal cells showed to be viable after attaching to CS/PCL scaffolds. The cell viability was high and the cells even proliferated on this scaffold. It is found that the CS/PCL fibrous scaffolds used in this study constitute a promising material for culturing these types of cells and for tissue engineering.
机译：通过混合天然和合成聚合物形成新颖的3D纤维支架是非常有趣的，并且对于许多生物医学应用而言很有希望。由壳聚糖和聚己内酯纤维制成的混合支架，由于结合了两种聚合物的优势，可以改善生物医学领域所需的机械，物理和生物学特性，因此对组织工程和再生医学应用具有很高的价值。为了制备PCL和CS混合物的电纺丝溶液，使用10：3 wt％的溶液并在用0.1 mm PTFE膜过滤后以20 kV的加速电压进行电纺丝。形态观察表明，纤维直径均匀（200-500nm）。差示扫描量热法和热重分析仪用于表征CS / PCL纳米纤维的热行为。此外，用人端粒化（hTERT）人骨髓（hBM）-间质（MSC）测试支架。将细胞以0.05x10（6）个细胞的密度接种在每个CS / PCL纳米纤维支架上，并在支架上培养6天。附着于CS / PCL支架后，骨髓基质细胞显示出活力。细胞活力高，甚至在该支架上增殖。发现本研究中使用的CS / PCL纤维支架构成了用于培养这些类型的细胞和用于组织工程的有前途的材料。

著录项

  • 来源
    《Science of advanced materials》 |2015年第11期|2427-2435|共9页
  • 作者

    Al Rez Mohammed Fayez; Fouad H.; Laourine Ezzedine; Hild Martin; Aibibu Dilbar; Cherif Chokri; Mahmood Amer; Abuelreich Sarah; Manikandan Muthurangan; Gosavi S. W.; Hashem Mohamed; Ansari S. G.; Al-Mubaddel Fahad S.; Elnakady Y. A.; Alqahtani Mahdi;

  • 作者单位

    King Saud Univ, Coll Appl Med Sci, Biomed Technol Dept, Riyadh 11466, Saudi Arabia;

    King Saud Univ, Coll Appl Med Sci, Biomed Technol Dept, Riyadh 11466, Saudi Arabia|Helwan Univ, Fac Engn, Dept Biomed Engn, Helwan, Egypt;

    Tech Univ Dresden, Inst Text Machinery & High Performance Mat Techno, D-01062 Dresden, Germany;

    Tech Univ Dresden, Inst Text Machinery & High Performance Mat Techno, D-01062 Dresden, Germany;

    Tech Univ Dresden, Inst Text Machinery & High Performance Mat Techno, D-01062 Dresden, Germany;

    Tech Univ Dresden, Inst Text Machinery & High Performance Mat Techno, D-01062 Dresden, Germany;

    King Saud Univ, Coll Med, Dept Anat, Stem Cell Unit, Riyadh 11437, Saudi Arabia|King Khalid Univ Hosp, Riyadh 11437, Saudi Arabia;

    King Saud Univ, Coll Med, Dept Anat, Stem Cell Unit, Riyadh 11437, Saudi Arabia|King Khalid Univ Hosp, Riyadh 11437, Saudi Arabia;

    King Saud Univ, Coll Med, Dept Anat, Stem Cell Unit, Riyadh 11437, Saudi Arabia|King Khalid Univ Hosp, Riyadh 11437, Saudi Arabia;

    Savitribai Phule Pune Univ, Dept Phys, Ctr Adv Studies Mat Sci & Solid State Phys, Pune 411007, Maharashtra, India;

    King Saud Univ, Coll Appl Med Sci, Dept Dent Hlth, Riyadh 11437, Saudi Arabia;

    Jamia Millia Islamia, Ctr Interdisciplinary Res Basic Sci, New Delhi 110025, India;

    King Saud Univ, Dept Chem Engn, Riyadh 11421, Saudi Arabia;

    King Saud Univ, Dept Zool, Coll Sci, Riyadh 11415, Saudi Arabia;

    King Saud Univ, Coll Appl Med Sci, Biomed Technol Dept, Riyadh 11466, Saudi Arabia;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    PCL; Chitosan; Electrospinning; DSC; FTIR; Mechanical Behavior; Bone Marrow Mesenchymal Stem Cells;

    机译：PCL;壳聚糖;静电纺丝;DSC;FTIR;力学行为;骨髓间充质干细胞;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号