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Seasonal and Cultivar Variation of Catechin in North East Indian Tea

机译：东北印度茶中儿茶素的季节性和品种变化

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摘要

ABSTRACT : Catechins are the major contributor to black tea quality attributes. They are precursors of theaflavin (TF) and thearubigin (TR) contributing to colour, astringency and strength of black tea. Present study is an attempt for biochemical fingerprinting of Assam, China and Cambod variety based on catechin composition and levels of oxidative enzymes, polyphenol oxidase (PPO) and peroxidase (PO). Seasonal variation of catechins and these enzymes was also studied. Estimation of catechin and the enzyme activity were carried out in High Performance Liquid Chromatography (HPLC) and Spectrophotometer respectively. Catechins were the highest in Assam variety followed by Cambod and China. Trihydroxy catechins accounted for 71-76% followed by dihydroxy 22-27% of total catechins in all the three varieties. Contribution of epigallocatechins gallate (EGCG) was 52-58% of total catechin. The EGCG content of the varieties were 121.68 ± 2.44 g/kg for Assam, 112.63 ± 2.90 g/kg for Cambod and 86.17 ± 1.32 g/kg for China. Distinct seasonal variation in EGCG, EC, total catechin and PO activity was observed.
机译：摘要：儿茶素是红茶品质属性的主要贡献者。它们是茶黄素（TF）和茶红素（TR）的前体，有助于红茶的颜色，涩味和强度。目前的研究是基于儿茶素的组成和氧化酶，多酚氧化酶（PPO）和过氧化物酶（PO）含量对阿萨姆邦和中国Cambod品种进行生化指纹识别的尝试。还研究了儿茶素和这些酶的季节性变化。儿茶素和酶活性的估计分别在高效液相色谱（HPLC）和分光光度计中进行。儿茶素在阿萨姆邦品种中含量最高，其次是柬埔寨和中国。在所有三个品种中，三羟基儿茶素占总儿茶素的71-76％，其次是二羟基22-7％。表没食子儿茶素没食子酸酯（EGCG）的贡献占儿茶素总量的52-58％。该品种的EGCG含量对阿萨姆邦为121.68±2.44 g / kg，对Cambod为112.63±2.90 g / kg，对中国而言为86.17±1.32 g / kg。观察到EGCG，EC，总儿茶素和PO活性的明显季节性变化。

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