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Phosphatase Activity of Aspergillus niger: A Native Tea Rhizosphere Isolate

机译：黑曲霉的磷酸酶活性：天然茶根际分离株。

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摘要

Phosphate solubilizing microbes are very important in any soil as well as in tea gardens. The isolates, viz. MM PSM10 (fungi) from a commercial tea estate of Upper Assam were used to study the enzyme activity. After screening of fourteen isolates from different locations MMPSM 10 was found to have phosphate solubilizing activity as evidenced by measuring the P -solubilization zone (3mm) in solid Pikovaskaya's medium after 4 days of incubation at 28°C ± 2°. The isolate recorded alkaline as well as acidic phosphatase activity. The experiment was conducted for 24, 48, 72 and 96 hour incubated cultures grown in nutrient broth at 30°C ± 2°. The Specific Activity was expressed as p-nitrophenol liberated (mM)/hr/mg of protein. MM PSM 10 (Aspergillus niger Code 1228.07 NCFT) revealed an increasing trend of alkaline phosphatase specific activity during its incubation period from initial 24 hours culture (2.66 ± 0.04) to 96 hours culture (5.24 ± 0.06). From the results MM PSM 10 proved to be an alternative for increased yield by solubilizing the bound phosphate, which may be present in soil or applied as superphosphate. Hence, the use of native isolate MM PSM 10 will improve the phosphate nutrition of the tea soil and plant in an economic and eco-friendly way.
机译：磷酸盐增溶微生物在任何土壤以及茶园中都非常重要。分离物，即。使用来自上阿萨姆邦商业茶园的MM PSM10（真菌）研究酶活性。在筛选了来自不同位置的14种分离株后，发现MMPSM 10具有磷酸盐增溶活性，这是通过在28°C±2°下温育4天后在固体Pikovaskaya's介质中测量P-增溶区（3毫米）来证明的。该分离物记录了碱性和酸性磷酸酶活性。实验进行了在营养肉汤中30°C±2°C下生长的24、48、72和96小时培养的培养物。比活度表示为释放的对硝基苯酚（mM）/ hr / mg的蛋白质。 MM PSM 10（黑曲霉代码1228.07 NCFT）显示了从最初的24小时培养（2.66±0.04）到96小时培养（5.24±0.06）的孵育期间，碱性磷酸酶比活性的增加趋势。根据结果​​，MM PSM 10被证明可以通过溶解结合的磷酸盐来提高产量，磷酸盐可以存在于土壤中或以过磷酸盐的形式使用。因此，使用天然分离物MM PSM 10将以经济和生态友好的方式改善茶土和植物的磷酸盐营养。

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