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首页> 外文期刊>Science and Culture >In Vitro Plant Regeneration From Mature explant Of Aegle Marmelos Corr.) Cv. cish-b2
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In Vitro Plant Regeneration From Mature explant Of Aegle Marmelos Corr.) Cv. cish-b2

机译：Aegle Marmelos Corr。的成熟外植体的体外植物再生。 cish-b2

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摘要

A protocol for rapid clonal micropropagation of Aegle marmelos (L.) Corr. cv. CISH-B2 was developed by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10- 15th nodal region of shoots in the second week of September gave quick in vitro bud burst (7.0 days) when cultured on MS medium supplemented with BAP 2.0 +IAA 1.0 mg/l. The maximum number of proliferated shoots (9.33 shoots/explant) were obtained on same medium supplemented with BAP 2.0 +IAA 1.0 mg/l. The micro shoots were rooted (95%) on Vi strength MS medium supplemented with IBA 10+IAA 1.0 mg/l. In vitro rooted plants were acclimatized on autoclaved coconut husk containing Vi strength MS plant salt mixture and under shade net house (50% shade 70%±5% RH). The plants were established in the field after acclimatization. The micropropagated plants were tested for its genetic fidelity by all the three Single Primer Amplification Reaction (SPAR) technique (13 RAPD, 3 ISSR and 2 DAMD primers) from mother tree and micropropagated plants.
机译：一种快速克隆微繁殖的Aegle marmelos（L.）Corr。简历。 CISH-B2是由成熟轴承树的节茎节开发而成的。当在补充有BAP 2.0 + IAA 1.0 mg / l的MS培养基上进行培养时，9月第二周从芽的第10-15个节点区域切下的一个长有芽的三厘米长芽具有快速的体外芽破裂（7.0天）。在补充了BAP 2.0 + IAA 1.0 mg / l的相同培养基上获得了最大的增生芽数（9.33个芽/外植体）。将微芽生根（95％）在补充有IBA 10 + IAA 1.0mg / l的Vi强度MS培养基上。使体外生根的植物在含有Vi强度MS植物盐混合物的高压灭菌的椰子壳上并在遮荫网箱（50％遮荫70％±5％RH）下适应。驯化后在田间建立了植物。通过来自母树和微繁殖植物的所有三种单引物扩增反应（SPAR）技术（13 RAPD，3 ISSR和2 DAMD引物）测试了微繁殖植物的遗传保真度。

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