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首页> 外文期刊>Science in China. Series C, Life sciences >Establishment of the model of white blood cell membrane chromatography and screening of antagonizing TLR_4 receptor component from Atractylodes macrocephala Koidz
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Establishment of the model of white blood cell membrane chromatography and screening of antagonizing TLR_4 receptor component from Atractylodes macrocephala Koidz

机译：白细胞膜色谱模型的建立及拮抗白术TLR_4受体成分的筛选

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摘要

A model of white blood cell membrane chromatography (WB-CMC) was established to screen active component from Atractylodes macrocephala Koidz. The component can antagonize Toll-like receptor 4 (TLR_4) and inhibit inflammatory reaction. In the model of WB-CMC, cell membrane stationary phase (CMSP) was prepared by immobilizing the rabbit white blood cell membrane (WBCM) onto the surface of silica carrier and taxinol was used as a model molecule. The active component which can act on WBCM and its receptor (such as TLR_4) as an effective target in A. macrocephala was determined by using a replacement experiment. The anti-inflammatory effects of the active component were tested by using pharmacological methods in vivo. The results indicated that the retention characteristics of atractylenolide I as active component was similar to that of taxinol in the model of WB-CMC. And so, atractylenolide I acted on the WBCM and TLR_4 and its anti-inflammatory activity was related with antagonizing TLR_4. Therefore, the interaction between the active component and WBCM and its receptor can be simulated by the model of WB-CMC in vitro. This model can be used to screen active components and to study effective characteristics for acting on definite targets.
机译：建立了白细胞膜色谱（WB-CMC）模型以筛选白术中的活性成分。该成分可以拮抗Toll样受体4（TLR_4）并抑制炎症反应。在WB-CMC模型中，通过将兔白细胞膜（WBCM）固定在二氧化硅载体表面上来制备细胞膜固定相（CMSP），并且紫杉醇用作模型分子。通过替代实验确定了可以作为大头曲霉有效靶标的WBCM及其受体（如TLR_4）起作用的活性成分。通过体内药理学方法测试了活性成分的抗炎作用。结果表明，在WB-CMC模型中，白术内酯I作为活性成分的保留特性与紫杉醇相似。因此，白术内酯I作用于WBCM和TLR_4，其抗炎活性与拮抗TLR_4有关。因此，可以通过WB-CMC体外模型来模拟活性成分与WBCM及其受体之间的相互作用。该模型可用于筛选活性成分并研究有效作用于确定目标的特征。

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