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Layer-by-layer assembly of enzymes and polymerized mediator on electrode surface by electrostatic adsorption

机译：酶和聚合介体通过静电吸附在电极表面的逐层组装

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Through layer-by-layer adsorption (LBL) technique, multilayer film was prepared from enzymes, diaphorase (DI) and glucose-6-phosphate dehydrogenase (G6PDH) and polyelectrolytes. The adsorption interface morphology was directly observed by atomic force microscopy, and the immobilization amount and layer thickness were characterized from quartz crystal microbalance which showed formation of nanoscale multilayer structure and linear mass increase which depended on alternate adsorption cycles. In order to construct a new mediated bi-enzyme biosensor system, polymerized mediator, DI and G6PDH were immobilized on carbon electrode surface by using LBL method. Electrochemical experiments indicated highly efficient electron transfer by the polymerized mediator. Two enzymes kept their activities after immobilization, and the electrode immobilized by mediator and enzymes showed sensitive response to glucose-6-phosphate in the presence of free NAD~+, and high stability during long period of storage.
机译：通过逐层吸附（LBL）技术，由酶，心肌黄递酶（DI），葡萄糖-6磷酸脱氢酶（G6PDH）和聚电解质制备了多层膜。通过原子力显微镜直接观察到吸附界面的形态，并通过石英晶体微天平来表征固定量和层厚，该晶体显示出纳米级多层结构的形成和线性质量的增加，这取决于交替的吸附循环。为了构建新的介导的双酶生物传感器系统，采用LBL法将聚合的介体DI和G6PDH固定在碳电极表面。电化学实验表明，聚合介体能高效转移电子。两种酶在固定化后仍保持活性，在游离NAD〜+存在下，介体和酶固定化的电极对6-磷酸葡萄糖有敏感的反应，并且在长期储存中具有很高的稳定性。

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