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首页> 外文期刊>Science and Technology of Advanced Materials >Functional analysis of phytochelatin synthase from Arabidopsis thaliana and its expression in Escherichia coli and Saccharomyces cerevisiae
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Functional analysis of phytochelatin synthase from Arabidopsis thaliana and its expression in Escherichia coli and Saccharomyces cerevisiae

机译：拟南芥中植物螯合素合酶的功能分析及其在大肠杆菌和酿酒酵母中的表达

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摘要

Plants, like other organisms, have adaptive mechanisms whereby they are able to respond to both nutrient deficiencies and toxicities. Phytochelatins (PCs) play an essential role in heavy-metal detoxification in plants, fungi and worms. PCs chelate heavy metals and then PC-metal complexes are translocated across the tonoplast and sequestered in vacuoles. PCs are synthesized from glutathione by the enzyme PC synthase (PCS). Comparison of the deduced amino acid sequences of PCS suggests that the C-terminal domain may be important for activation of the enzyme. We established the method for purification of PCS from Arabidopsis thaliana to perform enzymatic characterization. Moreover, the PCS gene was expressed in E. coli and S. cerevisiae to enhance tolerance to toxicity of cadmium ion. The obtained results implied that the some regions of the PCS may serve as regulatory region through interaction with cadmium ion and/or oxygen related compounds. Moreover, PCS expression dramatically enhanced cadmium ion tolerance of different organisms. Based on our findings, functional mechanism for PCS activation was hypothesized.
机译：像其他生物一样，植物具有适应性机制，因此它们能够对营养缺乏和毒性做出反应。植物螯合素（PCs）在植物，真菌和蠕虫的重金属排毒中起重要作用。 PC螯合重金属，然后PC金属配合物跨过液泡膜，并被隔离在液泡中。 PC是由PC合成酶（PCS）从谷胱甘肽合成的。 PCS推导的氨基酸序列的比较表明，C末端结构域可能对酶的激活很重要。我们建立了从拟南芥中纯化PCS的方法，以进行酶促表征。此外，PCS基因在大肠杆菌和酿酒酵母中表达，以增强对镉离子毒性的耐受性。获得的结果暗示PCS的一些区域可以通过与镉离子和/或氧相关化合物的相互作用而充当调节区域。而且，PCS表达显着增强了不同生物体对镉离子的耐受性。根据我们的发现，假设了PCS激活的功能机制。

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