• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Science and Technology of Advanced Materials >Microbial synthesis and enzymatic degradation of renewable poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate]
【24h】

Microbial synthesis and enzymatic degradation of renewable poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate]

机译：可再生聚[（R）-3-羟基丁酸酯-co-（R）-3-羟基己酸酯]的微生物合成和酶促降解

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This paper reports our recent progress on a microbial system for efficient production of biodegradable copolyester of poly[(P)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate], P(3HB-co-3HHx), with desirable copolymer compositions using genetically engineered bacteria. We have developed a fermentation technique to achieve high cell density cultivation of a recombinant Ralstonia eutropha using inexpensive soybean oil as a sole carbon source. In addition, we demonstrate that the use of polyhydroxyalkanoate (PHA) synthase (PhaC) mutants having an amino acid substitutions is able to vary the copolymer composition of P(3HB-co-3HHx) synthesized from soybean oil by the recombinant bacteria. On the other hand, it is also important to understand the enzymatic degradation process of PHA materials for establishing a method to regulate the rate of biodegradation. The enzymatic degradation process of P(3HB-co-3HHx) thin film using an extracellular PHB depolymerase has been studied by in situ atomic force microscopy in buffer solution. We demonstrate that the PHA depolymerase predominantly degrades the less-ordered molecular chain-packing regions along the crystallographic a-axis.
机译：本文报告了我们最近在有效生产可生物降解的聚[（P）-3-羟基丁酸酯-co-（R）-3-羟基己酸酯]，P（3HB-co-3HHx）和所需共聚物的共聚聚酯微生物系统方面的进展。使用基因工程细菌的组合物。我们已经开发出一种发酵技术，可利用廉价的大豆油作为唯一碳源，实现高重组重组拟南芥的高细胞密度培养。此外，我们证明使用具有氨基酸取代的聚羟基链烷酸酯（PHA）合酶（PhaC）突变体能够改变重组细菌从大豆油合成的P（3HB-co-3HHx）的共聚物组成。另一方面，理解PHA材料的酶促降解过程对于建立调节生物降解速率的方法也很重要。通过原位原子力显微镜在缓冲溶液中研究了使用细胞外PHB解聚酶对P（3HB-co-3HHx）薄膜的酶降解过程。我们证明PHA解聚酶主要降解沿结晶a轴的无序分子链堆积区域。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号