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首页> 外文期刊>The Science of the Total Environment >Reversibility of enzymatic reactions might limit biotransformation of organic micropollutants
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Reversibility of enzymatic reactions might limit biotransformation of organic micropollutants

机译：酶反应的可逆性可能会限制有机微量污染物的生物转化

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摘要

Biotransformation of many organic micropollutants (OMPs) in sewage treatment plants is incomplete leading to their release into the environment. Recent findings suggest that thermodynamic aspects of the reaction as chemical equilibrium limit biotransformation, while kinetic parameters have a lower influence. Reversibility of enzymatic reactions might result in a chemical equilibrium between the OMP and the transformation product, thus impeding a total removal of the compound. To the best of our knowledge, no study has focused on proving the reversible action of enzymes towards OMPs so far. Therefore, we aimed at demonstrating this hypothesis through in vitro assays with bisphenol A (BPA) in the presence of kinase enzymes, namely acetate kinase and hexokinase, which are key enzymes in anaerobic processes. Results suggest that BPA is phosphorylated by acetate kinase and hexokinase in the presence of ATP (adenosine 5-triphosphate), but when the concentration of this co-substrate decreases and the enzymes loss their activity, the backward reaction occurs, revealing a reversible biotransformation mechanism. This information is particularly relevant to address new removal strategies, which up to now were mainly focused on modifying the kinetic parameters of the reaction. (c) 2019 Elsevier B.V. All rights reserved.
机译：污水处理厂中许多有机微污染物（OMP）的生物转化不完全，导致其释放到环境中。最近的发现表明，作为化学平衡的反应的热力学方面限制了生物转化，而动力学参数具有较低的影响。酶促反应的可逆性可能会导致OMP与转化产物之间的化学平衡，从而阻碍了化合物的完全去除。据我们所知，到目前为止，还没有研究集中于证明酶对OMP的可逆作用。因此，我们旨在通过双酚A（BPA）在激酶酶（即乙酸激酶和己糖激酶）存在下的体外测定来证明这一假设，这是厌氧过程中的关键酶。结果表明，在存在ATP（5-三磷酸腺苷）的情况下，BPA被乙酸激酶和己糖激酶磷酸化，但是当该共底物的浓度降低且酶失去活性时，就会发生逆向反应，从而揭示了可逆的生物转化机制。该信息与解决新的去除策略特别相关，到目前为止，这些策略主要集中在修改反应动力学参数上。 （c）2019 Elsevier B.V.保留所有权利。

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