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首页> 外文期刊>The Science of the Total Environment >Quantitative proteomic analysis provides insights into the algicidal mechanism of Halobacillus sp. P1 against the marine diatom Skeletonema costatum
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Quantitative proteomic analysis provides insights into the algicidal mechanism of Halobacillus sp. P1 against the marine diatom Skeletonema costatum

机译：定量蛋白质组学分析提供了对Halobacillus sp。algicidalal机制的见解。 P1对海洋硅藻骨骼藻

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摘要

Algicidal behavior is a common interaction between marine microalgae and bacteria, especially in the dissipation phase of algal blooms. The marine bacterium Halobacillus sp. P1 was previously isolated and exhibits high algicidal activity against the diatom Skeletonema costatum. However, little is known about the mechanism underlying this algicidal process. Here, a tandem mass tag (TMT)-based proteomic approach was coupled with physiological analysis to investigate the cellular responses of S. costatum when treated with P1 culture supernatant. Among the 4582 proteins identified, 82 and 437 proteins were differentially expressed after treatment for 12 and 24 h, respectively. The proteomic results were in accordance with the results of verification by parallel reaction monitoring (PRM) assays. Proteins involved in reactive oxygen species scavenging, protein degradation and transport were upregulated, while proteins participating in nitrogen metabolism, protein translation, photosynthetic pigment biosynthesis and cell cycle regulation were significantly downregulated (p-value ≤0.05), corresponding to the increasing malondialdehyde content and the decreasing nitrogen, protein and chlorophyll a contents. A nutrient competitive relationship might exist between the bacterium P1 and S. costatum, and the inhibition of nitrogen metabolism by the P1 culture supernatant might be the key lethal factor that results in the dysfunction of S. costatum metabolism. Our study sheds light on the algicidal mechanism of PI at the molecular level and provides new insights into algae-bacteria interactions.
机译：杀藻行为是海洋微藻与细菌之间的常见相互作用，尤其是在藻华扩散期间。海洋细菌Halobacillus sp.。 P1以前是分离的，对硅藻骨骼藻表现出很高的杀藻活性。但是，对于这种藻灭过程的潜在机制知之甚少。在这里，基于串联质量标签（TMT）的蛋白质组学方法与生理分析相结合，以研究用P1培养上清液处理后肋骨链球菌的细胞反应。在处理的12和24小时中，鉴定出的4582种蛋白质中，分别有82和437个蛋白质差异表达。蛋白质组学结果与平行反应监测（PRM）分析的验证结果一致。参与活性氧清除，蛋白质降解和运输的蛋白质被上调，而参与氮代谢，蛋白质翻译，光合色素生物合成和细胞周期调控的蛋白质被显着下调（p值≤0.05），这与丙二醛含量和氮，蛋白质和叶绿素a含量降低。细菌P1和肋骨链球菌之间可能存在营养竞争关系，P1培养上清液对氮代谢的抑制可能是导致肋骨链球菌代谢功能障碍的关键致死因子。我们的研究在分子水平上揭示了PI的杀藻机制，并为藻类与细菌的相互作用提供了新的见解。

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