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首页> 外文期刊>The Science of the Total Environment >Characterisation of transcriptional responses to dioxins and dioxin-like contaminants in roach (Rutilus rutilus) using whole transcriptome analysis
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Characterisation of transcriptional responses to dioxins and dioxin-like contaminants in roach (Rutilus rutilus) using whole transcriptome analysis

机译：使用整个转录组分析表征蟑螂中对二恶英和二恶英类污染物的转录反应

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摘要

There is significant concern regarding the contamination of riverine sediments with dioxins and dioxin-like compounds (DLCs), including polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), polychlorinated biphenyls (PCBs) and some polycydic aromatic hydrocarbons (PAHs).The majority of studies investigating the ecotoxicology of DLCs in fish have focused on a few standard model species. However, there is significant uncertainty as to whether these model species are representative of native river fish, particularly in Europe. In this study, the transcriptional responses following exposure to equipotent concentrations of 23,7,8-tetrachlorodibenzo-p-dioxin (TCDD), PCB 156 or the dioxin-like PAH, benzo[K]fluoranthene (BkF), were investigated in juvenile roach (Rutihis run/us), a fish species that constitutes a large proportion of the fish biomass in freshwater bodies throughout Europe. To this end, RNA sequencing analysis was used to comprehensively characterise the molecular mechanisms and pathways of toxicity of these DLCs. Whole transcriptome analyses using ClueGO software revealed that DLCs have the potential to disrupt a number of important processes, including energy metabolism, oogenesis, the immune system, apoptosis and the response to oxidative stress. However, despite using equipotent concentrations, there was very little conservation of the transcriptional responses observed in fish exposed to different DLCs. TCDD provoked significant specific changes in the levels of transcripts related to immunotoxicity and carbohydrate metabolism, while PCB156 caused virtually no specific effects. Exposure to BkF affected the most diverse suite of molecular functions and biological processes, including blood coagulation, oxidative stress responses, unspecific responses to organic or inorganic substances/stimuli, cellular redox homeostasis and specific receptor pathways. To our knowledge, this is the first study of the transcriptome-wide effects of different classes of DLCs in fish. These findings represent an important step towards describing complete toxicity pathways of DLCs, which will be important in the context of informing risk assessments of DLC toxicity in native fish species.
机译：对于二恶英和二恶英类化合物（DLC）污染河流沉积物的问题，包括多氯二苯并对二恶英（PCDDs），多氯二苯并呋喃（PCDFs），多氯联苯（PCBs）和某些多环芳烃（PAHs）引起了人们的极大关注。 ）。研究鱼类中DLC的生态毒理学的大多数研究都集中在少数标准模型物种上。但是，关于这些模式物种是否代表本地河鱼，尤其是在欧洲，存在很大的不确定性。在这项研究中，在青少年中研究了当浓度为23,7,8-四氯二苯并-对-二恶英（TCDD），PCB 156或类似二恶英的PAH，苯并[K]荧蒽（BkF）后的转录反应。蟑螂（Rutihis run / us），一种鱼类，在整个欧洲的淡水中占鱼类生物量的很大一部分。为此，RNA测序分析被用来全面表征这些DLC的分子机制和毒性途径。使用ClueGO软件进行的整个转录组分析表明，DLC具有破坏许多重要过程的潜力，包括能量代谢，卵子发生，免疫系统，细胞凋亡以及对氧化应激的反应。然而，尽管使用了等电位的浓度，但是在暴露于不同DLC的鱼类中观察到的转录反应却几乎没有保留。 TCDD在与免疫毒性和碳水化合物代谢有关的转录水平上引起了显着的特异性变化，而PCB156几乎没有引起特异性作用。暴露于BkF会影响最广泛的分子功能和生物学过程，包括血液凝固，氧化应激反应，对有机或无机物质/刺激的非特异性反应，细胞氧化还原稳态和特定的受体途径。据我们所知，这是对鱼类中不同类别DLC的转录组范围内影响的首次研究。这些发现代表迈向描述DLC完整毒性途径的重要一步，这对告知本地鱼类DLC毒性风险评估至关重要。

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  • 来源
    《The Science of the Total Environment》 |2016年第15期|412-423|共12页
  • 作者

    Markus Brinkmann; Sven Koglin; Bryanna Eisner; Steve Wiseman; Markus Hecker; Kathrin Eichbaum; Beat Thalmann; Sebastian Buchinger; Georg Reifferscheid; Henner Hollert;

  • 作者单位

    Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany;

    Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany;

    Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada;

    Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada;

    Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada,School o/ the Environment & Sustainability, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada;

    Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany;

    Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany;

    Federal Institute of Hydrology (BfG), Department G3: Biochemistry, Ecotoxicology, Am Mainzer Tor 1, 56068 Koblenz, Germany;

    Federal Institute of Hydrology (BfG), Department G3: Biochemistry, Ecotoxicology, Am Mainzer Tor 1, 56068 Koblenz, Germany;

    Department of Ecosystem Analysis, Institute for Environmental Research, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany,College of Resources and Environmental Science, Chongqing University, 1 Tiansheng Road Beibei Chongqing 400715, China,College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, 1239 Siping Road, Shanghai China,State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Aryl hydrocarbon receptor; ClueGO; Illumina sequencing; Next-generation sequencing; RNAseq; Transcriptomics;

    机译：芳烃受体;线索GO;Illumina测序;下一代测序RNAseq;转录组学;

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