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首页> 外文期刊>The Science of the Total Environment >Nitration of pollen aeroallergens by nitrate ion in conditions simulating the liquid water phase of atmospheric particles
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Nitration of pollen aeroallergens by nitrate ion in conditions simulating the liquid water phase of atmospheric particles

机译：模拟大气颗粒液相的条件下硝酸根离子对花粉气敏原的硝化作用

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摘要

Pollen aeroallergens are present in atmospheric particulate matter (PM) where they can be found in coarse biological particles such as pollen grains (aerodynamic diameter d_(ae) ＞ 10 μm), as well as fragments in the finest re-spirable particles (PM2.5; d_(ae) ＜ 2.5 μm). Nitration of tyrosine residues in pollen allergenic proteins can occur in polluted air, and inhalation and deposition of these nitrated proteins in the human respiratory tract may lead to adverse health effects by enhancing the allergic response in population. Previous studies investigated protein nitration by atmospheric gaseous pollutants such as nitrogen dioxide and ozone. In this work we report, for the first time, a study on protein nitration by nitrate ion in aqueous solution, at nitrate concentrations and pH conditions simulating those occurring in the atmospheric aerosol liquid water phase. Experiments have been carried out on the Bovine serum albumin (BSA) protein and the recombinant Phleum pratense allergen (Phl p 2) both in the dark and under UV-A irradiation (range 4-90 W m~(-2)) to take into account thermal and/or photochemical nitration processes. For the latter protein, modifications in the allergic response after treatment with nitrate solutions have been evaluated by immunoblot analyses using sera from grass-allergic patients. Experimental results in bulk solutions showed that protein nitration in the dark occurs only in dilute nitrate solutions and under very acidic conditions (pH ＜ 3 for BSA; pH ＜ 2.2 for Phl p 2), while nitration is always observed (at pH 0.5-5) under UV-A irradiation, both in dilute and concentrated nitrate solutions, being significantly enhanced at the lowest pH values. In some cases, protein nitration resulted in an increase of the allergic response.
机译：花粉空气变应原存在于大气颗粒物（PM）中，在花粉颗粒（空气动力学直径d_（ae）＞ 10μm）等粗大的生物颗粒中会发现它们，在最细的可呼吸颗粒（PM2）中也会发现它们。 5； d_（ae）＜2.5μm）。花粉致敏蛋白中酪氨酸残基的硝化作用可能会在受污染的空气中发生，而这些硝化蛋白在人呼吸道中的吸入和沉积可能会通过增强人群的过敏反应而导致不良的健康影响。先前的研究调查了大气气体污染物（例如二氧化氮和臭氧）对蛋白质的硝化作用。在这项工作中，我们首次报告了在硝酸盐浓度和pH条件下模拟水溶液中硝酸盐离子在硝酸盐浓度和pH条件下硝化蛋白质的研究。已经在黑暗中和在UV-A照射下（4-90 W m〜（-2））对牛血清白蛋白（BSA）蛋白和重组and草变应原（Phl p 2）进行了实验，考虑到热和/或光化学硝化过程。对于后一种蛋白质，已经使用草过敏患者的血清通过免疫印迹分析评估了硝酸盐溶液处理后过敏反应的改变。大量溶液的实验结果表明，黑暗中的蛋白质硝化仅在稀硝酸盐溶液中和在非常酸性的条件下（BSA的pH ＜3； Phl p 2的pH ＜2.2）发生，而始终观察到硝化（pH值为0.5-5） ）在稀释的和浓硝酸盐溶液中的UV-A照射下，在最低pH值下均显着增强。在某些情况下，蛋白质硝化导致过敏反应增加。

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  • 来源
    《The Science of the Total Environment》 |2016年第15期|1589-1597|共9页
  • 作者

    Alessandra Ghiani; Maurizio Bruschi; Sandra Citterio; Ezio Bolzacchini; Luca Ferrero; Giorgia Sangiorgi; Riccardo Asero; Maria Grazia Perrone;

  • 作者单位

    Department of Earth and Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1,20126 Milan, Italy;

    Department of Earth and Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1,20126 Milan, Italy;

    Department of Earth and Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1,20126 Milan, Italy;

    Department of Earth and Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1,20126 Milan, Italy;

    Department of Earth and Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1,20126 Milan, Italy;

    Department of Earth and Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1,20126 Milan, Italy;

    Ambulatorio di Allergologia, Clinica San Carlo, Paderno Dugnano, Milan, Italy;

    Department of Earth and Environmental Sciences, University of Milano-Bicocca, P.zza della Scienza 1,20126 Milan, Italy;

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  • 正文语种 eng
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  • 关键词

    Acidic aerosol; DRH; UV-A irradiation; Tyrosine nitration; Allergenicity; Slot Blot;

    机译：酸性气雾剂DRH;UV-A照射;酪氨酸硝化;致敏性插槽印迹;

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