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首页> 外文期刊>The Science of the Total Environment >Abundance and diversity of methanogens: Potential role in high arsenic groundwater in Hetao Plain of Inner Mongolia, China
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Abundance and diversity of methanogens: Potential role in high arsenic groundwater in Hetao Plain of Inner Mongolia, China

机译：产甲烷菌的丰度和多样性：在内蒙古河套平原高砷地下水中的潜在作用

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摘要

To investigate the community diversity and abundance of methanogens and their potential role in high arsenic groundwater, 17 groundwater samples from Hetao Plain of Inner Mongolia were investigated with an integrated method including 16S rRNA gene clone library, quantitative polymerase chain reaction and geochemistry analyses. Total arsenic (As_(Tot)) concentrations were 82.7-1088.7 μg/L and arsenite (AsIII) mostly dominated in these samples with percentages of 0.04-0.79. CH_4 concentrations ranged from 0.01 to 292 μg/L and distinctly elevated only when As_(Tot) were relatively high and SO_4~(2-) were distinctly low. Principal component analysis indicated that these samples were divided into three groups according to the variations of As_(Tot). CH_4 and SO_4~(-2). As™ concentrations were distinctly high in the group with high CH_4 and low SO_4~(2-) comparing to the other two groups (one with high CH_4 and high SO_4~(2-), the other with low CH4 and SO_4~(2-)). The mcrA gene (methyl coenzyme-M reductase gene) based phylogenetic analysis of methanogens population showed that methanogenic archaea was diverse but mainly composed of Methanomicrobiales, Methanosarcinales, Methanobacteria and unidentified groups, with Methanomicrobiales being distinctly dominant (50.6%). The mcrA gene abundance in high arsenic ground-water ranged from 3.01 × 10~3 to 3.80 × 10~6 copies/L and accounted for 0-30.2% of total archaeal 16S rRNA genes. The abundance of mcrA genes was positively correlated with the concentrations of As_(Tot) (R = 0.59), AsIII (R = 0.57) and Fell (R = 0.79), while it was negatively correlated with oxidation-reduction potential (R = - 0.66) and SO_4~(2-) concentration (R = - 0.64). These results implied that methanogenic archaea might accelerate As release in groundwater aquifers in Hetao Plain.
机译：为了研究产甲烷菌的群落多样性和丰富性及其在高砷地下水中的潜在作用，采用包括16S rRNA基因克隆文库，定量聚合酶链反应和地球化学分析在内的综合方法，对内蒙古河套平原的17个地下水样品进行了调查。这些样品中的总砷（As_（Tot））浓度为82.7-1088.7μg/ L，其中主要以亚砷酸盐（AsIII）的百分比为0.04-0.79。 CH_4的浓度范围为0.01至292μg/ L，仅当As_（Tot）相对较高而SO_4〜（2-）明显较低时才明显升高。主成分分析表明，这些样品根据As_（Tot）的变化分为三类。 CH_4和SO_4〜（-2）。 CH_4高和SO_4〜（2-）低的组中的As™浓度显着高于其他两组（一个CH_4高和SO_4〜（2-）高的组，另一个CH4和SO_4〜（2）低的组-））。基于mcrA基因（甲基辅酶M还原酶基因）的产甲烷菌种群系统发育分析表明，产甲烷菌的古菌种类繁多，但主要由甲烷菌，甲烷菌，甲烷菌和未鉴定的群体组成，其中甲烷菌明显占优势（50.6％）。高砷地下水中的mcrA基因丰度范围为3.01×10〜3至3.80×10〜6拷贝/ L，占古细菌16S rRNA基因总数的0-30.2％。 mcrA基因的丰度与As_（Tot）（R = 0.59），AsIII（R = 0.57）和Fell（R = 0.79）的浓度呈正相关，而与氧化还原电位呈负相关（R =- 0.66）和SO_4〜（2-）浓度（R =-0.64）。这些结果表明，产甲烷的古细菌可能会加速河套平原地下水含水层中砷的释放。

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  • 来源
    《The Science of the Total Environment》 |2015年第may15期|153-161|共9页
  • 作者

    Y.H. Wang; P. Li; X.Y. Dai; R. Zhang; Z. Jiang; D.W. Jiang; Y.X. Wang;

  • 作者单位

    State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China;

    State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China;

    State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China;

    State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China;

    State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China,School of Environmental Studies, China University of Geosciences, Wuhan 430074, PR China;

    State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China;

    State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China,School of Environmental Studies, China University of Geosciences, Wuhan 430074, PR China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    mcrA; qPCR; Methanogen diversity; High arsenic groundwater; Inner Mongolia;

    机译：mcrA;定量PCR;产甲烷菌的多样性;高砷地下水;内蒙古;

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