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首页> 外文期刊>The Science of the Total Environment >A sensitive and accurate method for simultaneous analysis of algal toxins in freshwater using UPLC-MS/MS and ~(15)N-microcystins as isotopically labelled internal standards
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A sensitive and accurate method for simultaneous analysis of algal toxins in freshwater using UPLC-MS/MS and ~(15)N-microcystins as isotopically labelled internal standards

机译：用UPLC-MS / MS和〜（15）N-微囊蛋白同位素标记的内标在淡水中同时分析淡水中藻类毒素的敏感和准确方法

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摘要

The development of analytical methods for the detection and accurate quantification of algal toxins is of importance to assess the health risk of exposure to algal toxins in freshwater sources. This study established a sensitive and accurate analytical method for the quantification of 13 algal toxins (microcystins and nodularin) based on solid phase extraction (SPE) coupled with UPLC-MS/MS, in which ~(15)N-microcystins were used as surrogate/internal standards. SPE method was optimized to extract the target algal toxins in freshwater samples. Good SPE efficiencies (84-96%) were achieved for the overwhelming majority of the investigated algal toxins when SPE was performed using HLB (500 mg, 6 mL) under alkaline conditions (pH 11). An accurate quantitative analysis of the algal toxins in real freshwater samples was performed by using ~(15)N-labelled microcystins as isotopically labelled internal standards (ILISs), which compensated for the loss of target toxins during the whole analytical process. In addition. ILISs also helped to correct the effects of environmental matrices and instrument fluctuation in UPLC-MS/MS analysis. The limit of method quantification (MQL) for the algal toxins was ＜2.0 ng/L that is sensitive enough to quantify extremely low levels of target toxins in freshwater samples.
机译：用于检测和准确定量藻类毒素的分析方法的开发对于评估淡水来源暴露于藻类毒素的健康风险是重要的。本研究建立了基于与UPLC-MS / MS偶联的固相萃取（SPE）的13次藻类毒素（微囊藻蛋白和结节蛋白）的敏感和准确的分析方法，其中〜（15）使用〜（15）n-微囊蛋白作为替代/内部标准。优化SPE方法以提取淡水样品中的靶藻类毒素。当使用HLB（500mg，6ml）在碱性条件下（pH 11）下进行SPE时，对大多数研究的藻类毒素实现了良好的SPE效率（84-96％）。通过使用〜（15）n标记的微囊藻如同同位素标记的内标（Iliss）进行了实际淡水样品中的藻类毒素的精确定量分析，该内标在整个分析过程中补偿了靶毒素的丧失。此外。 Iliss还有助于纠正环境矩阵和仪器波动在UPLC-MS / MS分析中的影响。用于藻类毒素的方法定量（MQL）的极限为<2.0 ng / L，足以量化淡水样品中的极低水平的靶毒素。

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  • 来源
    《The Science of the Total Environment》 |2020年第10期|139727.1-139727.10|共10页
  • 作者

    Ngoc Han Tran; Yiwen Li; Martin Reinhard; Yiliang He; Karina Yew-Hoong Gin;

  • 作者单位

    Institute of Research and Development Duy Tan University Da Nang 550000 Viet Nam NUS Environmental Research Institute National University of Singapore 1 Create Way Create Tower #15-02 Singapore 138602 Singapore;

    Department of Environmental Science and Engineering Sichuan University China;

    Department of Civil and Environmental Engineering Stanford University CA 94305 USA;

    School of Environmental Science and Engineering Shanghai Jiao Tong University Shanghai 200240 China;

    NUS Environmental Research Institute National University of Singapore 1 Create Way Create Tower #15-02 Singapore 138602 Singapore Department of Civil and Environmental Engineering National University of Singapore 1 Engineering Drive 2 Singapore 117576 Singapore;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cyanotoxins; Drinking water sources; Harmful algal bloom; Microcystins; Nodularin;

    机译：青紫毒素;饮用水来源;有害的藻类绽放;微囊辛;结核病;

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