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首页> 外文期刊>Science of the total environment >SARS-CoV-2 RNA detection and persistence in wastewater samples: An experimental network for COVID-19 environmental surveillance in Padua, Veneto Region (NE Italy)
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SARS-CoV-2 RNA detection and persistence in wastewater samples: An experimental network for COVID-19 environmental surveillance in Padua, Veneto Region (NE Italy)

机译：废水样本中的SARS-COV-2 RNA检测和持久性：威尼托地区帕多瓦的Covid-19环境监测实验网络（Ne Italy）

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摘要

Background: Clinical detection of SARS-CoV-2 RNA in stools supports the idea of wastewater-based epidemiology (WBE) as a precious tool for COVID-19 environmental surveillance. Successful detection of SARS-CoV-2 RNA in untreated wastewaters has been reported in several countries. This study investigated the presence and persistence of viral RNA in treated and untreated wastewaters in Padua, Italy. An urban experimental network of sampling sites was tested for prospective surveillance activities. Methods: Seven sampling sites (i.e. wastewater pumping stations, plant inlets and outlets) were selected from the two main municipal wastewater treatment plant systems. Eleven grab samples (9 untreated, 2 treated wastewaters) were collected on 2 dates. All samples were tested at t0 for SARS-CoV-2 RNA and t1 = 24 h to investigate its persistence, at room temperature and under refrigerated conditions. Overall, 33 sub-samples were concentrated by ultrafiltration and tested for molecular detection of viral RNA with two RT-qPCR assays. Results: At t0, positivity for at least one RT-qPCR assay was achieved by 4/9 untreated wastewater samples and 2/ 2 tertiary treated samples. A minimum SARS-CoV-2 titer of 4.8-4.9 log10 gc/L was estimated. At tl, three refrigerated subsamples were positive as well. The two RT-qPCR assays showed differential sensitivity, with the N assay detecting 90% of successful amplifications. Conclusions: SARS-CoV-2 RNA was detected in untreated and treated wastewaters. Its persistence after 24 h was demonstrated in subsamples kept at 4°C. Hospitalization data suggested an approximate WBE detection power of 1 COVID-19 case per 531 inhabitants. The possible role of WBE in COVID-19 environmental surveillance is strongly supported by our findings. WBE can also provide precious support in the decision-making process of restriction policies during the epidemic remission phase. Optimization and standardization of laboratory methods should be sought in the short term, so that results from different studies can be compared with reliability.
机译：背景：SARS-COV-2 RNA在粪便中的临床检测支持废水的流行病学（WBE）作为Covid-19环境监测的珍贵工具。在几个国家据报道，在未处理的废水中成功检测在未处理的废水中的SARS-COV-2 RNA。本研究研究了意大利帕多瓦治疗和未处理的废水中病毒RNA的存在和持续性。测试了一个对预期监测活动的抽样网站的城市实验网络。方法：选自七个采样点（即废水泵站，植物入口和出口）选自两个主要的市政污水处理厂系统。在2个日期收集11个抓取样品（9个未处理的2个处理的废水）。在T0的SARS-COV-2 RNA和T1 = 24小时内测试所有样品，以在室温和冷藏条件下探讨其持久性。总体而言，通过超滤浓缩33个子样品，并测试具有两种RT-QPCR测定的病毒RNA的分子检测。结果：在T0，通过4/9未处理的废水样品和2/2叔处理的样品实现至少一个RT-QPCR测定的阳性。估计了4.8-4.9 LOG10 GC / L的最小SARS-COV-2滴度。在TL，三个冷藏副产品也是正的。两种RT-QPCR测定显示差异敏感性，n个测定检测90％的成功扩增。结论：在未处理和处理过的废水中检测到SARS-COV-2 RNA。 24小时后的持续性在4°C保持的子样品中展示。住院数据建议每531名居民1个Covid-19例的近似WBE检测力。 WBE在Covid-19环境监测中的可能作用得到了我们的研究结果。 WBE还可以在流行性缓解阶段在限制政策的决策过程中提供珍贵支持。应在短期内寻求实验室方法的优化和标准化，从而可以将不同研究的结果与可靠性进行比较。

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  • 来源
    《Science of the total environment》 |2021年第15期|143329.1-143329.7|共7页
  • 作者

    Tatjana Baldovin; Irene Amoruso; Marco Fonzo; Alessandra Buja; Vincenzo Baldo; Silvia Cocchio; Chiara Bertoncello;

  • 作者单位

    Department of Cardiac Thoracic Vascular Sciences and Public Health Unit of Hygiene and Public Health University of Padua Padua Italy;

    Department of Cardiac Thoracic Vascular Sciences and Public Health Unit of Hygiene and Public Health University of Padua Padua Italy;

    Department of Cardiac Thoracic Vascular Sciences and Public Health Unit of Hygiene and Public Health University of Padua Padua Italy;

    Department of Cardiac Thoracic Vascular Sciences and Public Health Unit of Hygiene and Public Health University of Padua Padua Italy;

    Department of Cardiac Thoracic Vascular Sciences and Public Health Unit of Hygiene and Public Health University of Padua Padua Italy;

    Department of Cardiac Thoracic Vascular Sciences and Public Health Unit of Hygiene and Public Health University of Padua Padua Italy;

    Department of Cardiac Thoracic Vascular Sciences and Public Health Unit of Hygiene and Public Health University of Padua Padua Italy;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    WBE; Environmental surveillance; SARS-CoV-2; COVID-19; Sewage;

    机译：WBE;环境监测;SARS-NO-2;NovID-19;污水;

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