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首页> 外文期刊>Science of the total environment >Selenium-oxidizing Agrobacterium sp. T3F4 steadily colonizes in soil promoting selenium uptake by pak choi (Brassica campestris)
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Selenium-oxidizing Agrobacterium sp. T3F4 steadily colonizes in soil promoting selenium uptake by pak choi (Brassica campestris)

机译：硒氧化土壤杆菌SP。 T3F4在土壤中稳步殖民促进硒菌（Brassica Campestris）促进硒吸收

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摘要

Selenium (Se) deficiency in soil is linked to its low content in edible crops, resulting in adverse impacts on the health of 15% of the global population. The crop mainly absorbs oxidized selenate and selenite from soil, then converts them into organic Se. However, the role of Se-oxidizing bacteria in soil Se oxidation, Se bioavailability and Se absorption into plants remains unclear. The strain Agrobacterium sp. T3F4, isolated from seleniferous soil, was able to oxidize elemental Se into selenite under pure culture conditions. The green fluorescent protein (gfp)-gene-marked strain (T3F4-GFP) and elemental Se or selenite (5 mg·kg~(-1)) were added to pak choi (Brassica campestris ssp. chinensis) pot cultures. Observation of the fluorescence and viable counting indicated that GFP-expressing bacterial cells steadily colonized the soil in the pots and the leaves of the pak choi, reaching up to 6.6 × 10~6 and 2.0 × 10~5 CFU g~(-1) at 21 days post cultivation, respectively. Moreover, the total Se content (mostly organic Se) was significantly increased in the pak choi under T3F4 inoculated pot culture, with elemental Se(0) being oxidized into Se(Ⅳ), and soil Se(Ⅳ) being dissolved before being absorbed by the crop. After strain T3F4 was inoculated, no significant differences in microbial diversity were observed in the soils and roots, whereas the abundance of Rhizobium spp. was significantly increased. To our knowledge, this is the first time that Se-oxidizing Agrobacterium sp. T3F4 has been found to steadily colonize soil and plant tissues, and that its addition to soil increases the absorption of Se in plants. This study provides a potential strategy for Se biofortification.
机译：土壤缺乏缺乏与食用作物中的低含量有关，导致对全球人口的15％的健康产生不利影响。作物主要吸收从土壤中氧化硒酸盐和硒酸盐，然后将它们转化为有机硒。然而，Se-氧化细菌在土壤Se氧化，SE生物利用度和Se吸收到植物中的作用仍然不清楚。菌株SPATROUMIUM SP。与硒土壤分离的T3F4能够在纯培养条件下将元素SE氧化成硒。将绿色荧光蛋白（GFP）标记的菌株（T3F4-GFP）和元素SE或硒沸石（5mg·Kg〜（-1））加入到Pak Choi（Brassica Campestris SSP中.Chensensis）pot培养物。观察荧光和可行计数表明，表达GFP的细菌细胞稳定地将土壤中的土壤殖民和Pak Choi的叶子，达到6.6×10〜6和2.0×10〜5 CFU G〜（-1）培养后21天。此外，在T3F4接种的罐培养下，Pak Choi中的Se含量（大多数有机Se）显着增加，用元素SE（0）被氧化成SE（Ⅵ），并在被吸收之前溶解土壤SE（ⅳ）作物。在接种菌株T3F4之后，在土壤和根部中没有观察到微生物多样性的显着差异，而Rhizobium SPP的丰度。显着增加。据我们所知，这是第一次氧化土壤杆菌SP。已经发现T3F4稳定地殖民殖民植物组织，并且其对土壤的添加增加了植物中SE的吸收。本研究为SE生物侵蚀提供了潜在的策略。

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  • 来源
    《Science of the total environment》 |2021年第15期|148294.1-148294.8|共8页
  • 作者

    Dahui Zhu; Yaxin Niu; Keke Fan; Fujun Zhang; Yu Wang; Gejiao Wang; Shixue Zheng;

  • 作者单位

    State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 PR China;

    State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 PR China;

    State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 PR China;

    State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 PR China;

    State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 PR China;

    State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 PR China;

    State Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 PR China;

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  • 正文语种 eng
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  • 关键词

    Selenium oxidation; Selenium solubilization; Selenium biofortification; Rhizosphere;

    机译：硒氧化;硒溶解;硒生物化;根际;

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