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首页> 外文期刊>Science of the total environment >Exploring untapped potential of Streptomyces spp. in Gurbantunggut Desert by use of highly selective culture strategy
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Exploring untapped potential of Streptomyces spp. in Gurbantunggut Desert by use of highly selective culture strategy

机译：探索链霉菌SPP的未开发潜力。 通过使用高度选择性的文化策略在Gurbantunggut沙漠中

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摘要

Streptomycetes have been, for over 70 years, one of the most abundant sources for the discovery of new antibiotics and clinic drugs. However, in recent decades, it has been more and more difficult to obtain new phylotypes of the genus Streptomyces by using conventional samples and culture strategies. In this study, we combined culture-dependent and culture-independent approaches to better explore the Streptomyces communities in desert sandy soils. Moreover, two different culture strategies termed Conventional Culture Procedure (CCP) and Streptomycetes Culture Procedure (SCP) were employed to evaluate the isolation efficiency of Streptomyces spp. with different intensities of selectivity. The 16S rRNA gene amplicon analysis revealed a very low abundance (0.04-0.37%, average 0.22%) of Streptomyces in all the desert samples, conversely the percentage of Streptomyces spp. obtained by the culture-dependent method was very high (5.20-39.57%, average 27.76%), especially in the rhizospheric sand soils (38.40-39.57%, average 38.99%). Meanwhile, a total of 1589 pure cultures were isolated successfully, dominated by Streptomyces (29.52%), Microvirga (8.06%) and Bacillus (7.68%). In addition, 400 potential new species were obtained, 48 of which belonged to the genus Streptomyces. More importantly, our study demonstrated the SCP strategy which had highly selectivity could greatly expand the number and phylotypes of Streptomyces spp. by almost 4-fold than CCP strategy. These results provide insights on the diversity investigation of desert Streptomyces, and it could be reference for researchers to bring more novel actinobacteria strains from the environment into culture.
机译：肌肉细胞已超过70年，发现新的抗生素和临床药物最丰富的源。然而，近几十年来，通过使用常规样品和培养策略，获得链霉菌的新农场型，越来越困难。在这项研究中，我们组合了文化依赖性和文化的途径，以更好地探索沙漠沙质土壤中的链霉菌社区。此外，使用常规培养程序（CCP）和链霉菌培养程序（SCP）的两种不同的培养策略来评估链霉菌SPP的分离效率。具有不同的选择性强度。 16S rRNA基因扩增子分析显示出所有沙漠样本中的高丰度（0.04-0.37％，平均0.22％），相反，Streptomyces SPP的百分比。通过培养依赖性方法获得非常高（5.20-39.57％，平均27.76％），特别是在脱菱砂土中（38.40-39.57％，平均为38.99％）。同时，共成功地分离了1589种纯培养物，以链霉菌（29.52％），微血管（8.06％）和芽孢杆菌（7.68％）为主。此外，获得了400个潜在的新物种，其中48种属于链霉菌属。更重要的是，我们的研究表明，具有高度选择性的SCP策略可以大大扩大链霉菌SPP的数量和线型。比CCP策略几乎4倍。这些结果提供了对沙漠链霉菌的多样性调查的见解，并且可以参考研究人员从环境中带来更多新的肌动菌菌株。

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  • 来源
    《Science of the total environment》 |2021年第10期|148235.1-148235.15|共15页
  • 作者

    Shuai Li; Lei Dong; Wen-Hui Lian; Zhi-Liang Lin; Chun-Yan Lu; Lu Xu; Li Li; Wael N. Hozzein; Wen-Jun Li;

  • 作者单位

    State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) School of Life Sciences. Sun Yat-Sen University Guangzhou 510275 PR China;

    State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) School of Life Sciences. Sun Yat-Sen University Guangzhou 510275 PR China;

    State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) School of Life Sciences. Sun Yat-Sen University Guangzhou 510275 PR China;

    State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) School of Life Sciences. Sun Yat-Sen University Guangzhou 510275 PR China;

    State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) School of Life Sciences. Sun Yat-Sen University Guangzhou 510275 PR China;

    State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) School of Life Sciences. Sun Yat-Sen University Guangzhou 510275 PR China;

    State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi 830011 PR China;

    Zoology Department College of Science King Saud University Riyadh 999088 Saudi Arabia Botany and Microbiology Department Faculty of Science Beni-Suef University Beni-Suef 62511 Egypt;

    State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) School of Life Sciences. Sun Yat-Sen University Guangzhou 510275 PR China State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences Urumqi 830011 PR China;

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  • 正文语种 eng
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  • 关键词

    Culture-dependent; Culture-independent; Streptomycetes culture procedure (SCP); Conventional culture procedure (CCP); Streptomyces spp.; Isolation efficiency;

    机译：文化依赖;无关;链霉菌培养程序（SCP）;常规培养程序（CCP）;Streptomyces spp;隔离效率;

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