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Moving Target

机译:移动目标

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摘要

The complex, ever-changing multitude of proteins present in a cell or tissue at any given time, referred to as the proteome, is difficult to capture and describe. Molecular biologists have a variety of tools at their disposal to study proteins, but arguably none is more powerful for the comprehensive analysis of proteins present in a complex sample than mass spectrometry. Traditionally, researchers have used one of two mass spec-based approaches to tease apart the complexities of the proteome: a discovery-based strategy to identify as many proteins as possible, and a targeted approach to accurately quantify a few select proteins of interest.
机译:在任何给定时间存在于细胞或组织中的复杂,千变万化的蛋白质(称为蛋白质组)很难捕获和描述。分子生物学家可以使用多种工具来研究蛋白质,但是可以说,没有任何一种工具能够比质谱法更全面地分析复杂样品中存在的蛋白质。传统上,研究人员使用两种基于质谱的方法中的一种来弄清蛋白质组的复杂性:一种基于发现的策略,以识别尽可能多的蛋白质,以及一种针对性的方法,以准确地定量一些目标蛋白。

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