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首页> 外文期刊>Sensors and Actuators >Enhanced determination of fertility hormones in saliva at disposable immunosensing platforms using a custom designed field-portable dual potentiostat
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Enhanced determination of fertility hormones in saliva at disposable immunosensing platforms using a custom designed field-portable dual potentiostat

机译：使用定制设计的现场便携式双电位ostat增强一次性免疫抑制平台的唾液中生育荷尔蒙的测定

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摘要

This work describes a new electroanalytical device for the simultaneous and reliable determination of two fertility relevant hormones (luteinizing hormone, LH, and progesterone, P4) in saliva. The device is constructed using a custom designed and field-portable potentiostat where dual disposable immunosensing platform are connected. The immunosensors are based on sandwich-type and competitive immunoassays implemented onto magnetic microbeads (MBs) functionalized with Neutravidin and Protein G for the determination of LH and P4, respectively. Amperometric detection performed at - 0.20 V vs the Ag pseudo-reference electrode using the H2O2/hydroquinone (HQ) system was employed as the transduction technique after placing the MBs with immunocomplexes for each target hormone on the appropriate working electrode of screen-printed dual carbon electrodes (SPdCEs). The method exhibits high sensitivity and selectivity for the target hormones providing detection limits of 1.7 pg mL(-1) and 0.10 mIU mL(-1) for P4 and LH, respectively, with a 1 h test time. The applicability of the method was confirmed by determining both hormones in saliva samples from different volunteers providing results comparable to those obtained using amperometric transduction with a commercial potentiostat and with ELISA methodologies involving the same immunoreagents for each target hormone.
机译：这项工作描述了一种新的电解理装置，用于同时可靠地测定唾液中的两种生育率相关激素（叶氏素激素，LH和孕酮，P4）。该装置是使用定制设计的和现场便携的电位ostat构造的，其中连接双倍一次性免疫抑制平台。免疫传感器基于夹层型和竞争性免疫测定在用中性杀素和蛋白G官能化的磁性微珠（MBS）上，分别用于测定LH和P4。使用H 2 O 2 /氢醌（HQ）系统在0.20V VS的Ag伪参考电极在丝网印刷的双碳的适当工作电极上将MB与免疫混合物放置在丝网印刷的双碳的适当工作电极上的免疫混合物之后，使用 - 0.20V VS型Ag伪参考电极。电极（SPDCES）。该方法对P4和LH的检测限为1.7pg ml（-1）和0.10mL（-1）的靶激素具有高灵敏度和选择性，具有1小时的测试时间。通过从不同志愿者确定唾液样本中的唾液中的两个激素来证实该方法的适用性，提供与使用商业诱发仪和涉及每个靶激素的相同免疫造成的ELISA方法获得的结果相当的结果。

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  • 来源
    《Sensors and Actuators》 |2019年第11期|126934.1-126934.9|共9页
  • 作者

    Serafin V.; Arevalo B.; Martinez-Garcia G.; Aznar-Poveda J.; Lopez-Pastor J. A.; Beltran-Sanchez J. F.; Garcia-Sanchez A. J.; Garcia-Haro J.; Campuzano S.; Yanez-Sedeno P.; Pingarron J. M.;

  • 作者单位

    Univ Complutense Madrid Fac Chem Dept Analyt Chem Ciudad Univ S-N E-28040 Madrid Spain;

    Univ Complutense Madrid Fac Chem Dept Analyt Chem Ciudad Univ S-N E-28040 Madrid Spain;

    Univ Complutense Madrid Fac Chem Dept Analyt Chem Ciudad Univ S-N E-28040 Madrid Spain;

    Tech Univ Cartagena ETSIT Dept Informat & Commun Technol TIC Campus Muralla Mar E-30202 Cartagena Spain;

    Tech Univ Cartagena ETSIT Dept Informat & Commun Technol TIC Campus Muralla Mar E-30202 Cartagena Spain;

    Tech Univ Cartagena ETSIT Dept Informat & Commun Technol TIC Campus Muralla Mar E-30202 Cartagena Spain;

    Tech Univ Cartagena ETSIT Dept Informat & Commun Technol TIC Campus Muralla Mar E-30202 Cartagena Spain;

    Tech Univ Cartagena ETSIT Dept Informat & Commun Technol TIC Campus Muralla Mar E-30202 Cartagena Spain;

    Univ Complutense Madrid Fac Chem Dept Analyt Chem Ciudad Univ S-N E-28040 Madrid Spain;

    Univ Complutense Madrid Fac Chem Dept Analyt Chem Ciudad Univ S-N E-28040 Madrid Spain;

    Univ Complutense Madrid Fac Chem Dept Analyt Chem Ciudad Univ S-N E-28040 Madrid Spain;

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  • 正文语种 eng
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  • 关键词

    Dual immunosensor; Fertility hormones; Magnetic beads; Dual immunosensor; Saliva; Field-portable potentiostat;

    机译：双免疫传感器;生育荷尔蒙;磁珠;双免疫传感器;唾液;现场便携式电位塔;

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