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首页> 外文期刊>Sensors and Actuators >Protect to detect: A Golgi apparatus targeted probe to image mobile zinc through the use of a lipophilic cell-labile protecting group strategy
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Protect to detect: A Golgi apparatus targeted probe to image mobile zinc through the use of a lipophilic cell-labile protecting group strategy

机译：防止检测：通过使用亲脂性细胞 - 不稳定保护组策略来检测GOLGI装置对图像移动锌的探头

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摘要

The Golgi apparatus requires zinc for its normal function, but the role it plays in these processes at the sub-cellular level is not well-understood due to the lack of appropriate tools to image it. Herein, a small molecule Golgi apparatus targeted probe was developed to image mobile Zn~(2+). A trityl group was used to protect a Golgi apparatus targeting cysteine residue to increase cell membrane permeability, which was then removed within 24 h, revealing the free cysteine targeting motif to anchor the probe to the Golgi apparatus. The probe shows good photophysical properties, good selectivity and Zn~(2+) response over a wide range of pH as well as low cellular toxicity. The probe was shown to be capable of targeting the Golgi apparatus and responding to Zn~(2+) in a number of different cell lines and was also applied to monitor the change of concentration of mobile Zn~(2+) in the Golgi apparatus in response to oxidative stress.
机译：Golgi设备需要锌的正常功能，但由于缺乏缺乏适当的工具来缺乏缺乏映像，它在子蜂窝水平下的这些过程中扮演的作用是不太理解的。 在此，开发了一种小分子GOLGI装置靶向探针，用于图像移动Zn〜（2+）。 使用Trityl组来保护靶向半胱氨酸残基的Golgi装置以增加细胞膜渗透性，然后在24小时内除去，揭示自由半胱氨酸靶向基序，将探针锚定向高尔基装置。 探针显示出良好的光学性质，良好的选择性和Zn〜（2+）在各种pH和低细胞毒性方面的响应。 探针被证明能够靶向GOLGI装置并在许多不同的细胞系中响应Zn〜（2+），并且还应用于监测GOLGI装置中的移动Zn〜（2+）的浓度变化 响应于氧化应激。

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  • 来源
    《Sensors and Actuators》 |2021年第7期|129850.1-129850.10|共10页
  • 作者

    Le Fang; Rachel Crespo-Otero; Christopher R. Jones; Michael Watkinson;

  • 作者单位

    The Joseph Priestley Building School of Biological and Chemical Science Queen Mary University of London Mile End Road London E1 4NS UK;

    The Joseph Priestley Building School of Biological and Chemical Science Queen Mary University of London Mile End Road London E1 4NS UK;

    The Joseph Priestley Building School of Biological and Chemical Science Queen Mary University of London Mile End Road London E1 4NS UK;

    The Lennard-Jones Laboratories School of Chemical and Physical Science Keele University ST5 5BG UK;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Golgi apparatus targeting; Fluorescent probe; Zinc imaging; Click chemistry; Oxidative stress;

    机译：高尔基设备瞄准;荧光探针;锌成像;点击化学;氧化应激;

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