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首页> 外文期刊>Sensors and Actuators >Rapid point-of-care testing for SARS-CoV-2 virus nucleic acid detection by an isothermal and nonenzymatic Signal amplification system coupled with a lateral flow immunoassay strip
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Rapid point-of-care testing for SARS-CoV-2 virus nucleic acid detection by an isothermal and nonenzymatic Signal amplification system coupled with a lateral flow immunoassay strip

机译：SARS-COV-2病毒核酸检测的快速护理检测由等温和非酶信号放大系统与侧向流动免疫测定带进行耦合

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摘要

An outbreak of a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), began in December 2019. Accurate, rapid, convenient, and relatively inexpensive diagnostic methods for SARS-CoV-2 infection are important for public health and optimal clinical care. The current gold standard for diagnosing SARS-CoV-2 infection is reverse transcription-polymerase chain reaction (RT-PCR). However, RTPCR assays are designed for use in well-equipped laboratories with sophisticated laboratory infrastructure and highly trained technicians, and are unsuitable for use in under-equipped laboratories and in the field. In this study, we report the development of an accurate, rapid, and easy-to-implement isothermal and nonenzymatic signal amplification system (a catalytic hairpin assembly (CHA) reaction) coupled with a lateral flow immunoassay (LFIA) strip-based detection method that can detect SARSCoV-2 in oropharyngeal swab samples. Our method avoids RNA isolation, PCR amplification, and elaborate result analysis, which typically takes 6-8 h. The entire CHA-LFIA detection method, from nasopharyngeal sampling to obtaining test results, takes less than 90 min. Such methods are simple and require no expensive equipment, only a simple thermostatically controlled water bath and a fluorescence reader device. We validated our method using synthetic oligonucleotides and clinical samples from 15 patients with SARS-CoV-2 infection and 15 healthy individuals. Our detection method provides a fast, simple, and sensitive (with a limit of detection (LoD) of 2000 copies/mL) alternative to the SARS-CoV-2 RT-PCR assay, with 100 % positive and negative predictive agreements.
机译：2019年12月开始爆发新的冠状病毒，严重的急性呼吸综合征冠状病毒2（SARS-COV-2）。SARS-COV-2感染的准确，快速，方便，相对便宜，廉价的诊断方法对公共卫生以及最佳临床护理。目前诊断SARS-COV-2感染的金标准是逆转录 - 聚合酶链反应（RT-PCR）。然而，RTPCR测定设计用于设备齐全的实验室，具有精致的实验室基础设施和高度训练有素的技术人员，并且不适合在设备下的实验室和现场使​​用。在这项研究中，我们报告了耦合的准确，快速且易于实施的等温和非酶信号放大系统（催化发夹组件（CHA）反应），其与侧向流动免疫测定（LFIA）基于基于条带的检测方法偶联这可以检测口咽拭子样本中的SARSCOV-2。我们的方法避免了RNA分离，PCR扩增和精细结果分析，其通常需要6-8小时。从鼻咽采样获得测试结果的整个CHA-LFIA检测方法，需要小于90分钟。这种方法简单，不需要昂贵的设备，只有简单的恒温控制水浴和荧光读取器装置。我们使用来自15名SARS-COV-2感染和15名健康个体的患者使用合成寡核苷酸和临床样本的方法验证了我们的方法。我们的检测方法提供了快速，简单，敏感的（具有2000份/ mL的检测（LOD）的极限，替代SARS-COV-2 RT-PCR测定，具有100％的阳性和负面预测协议。

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  • 来源
    《Sensors and Actuators》 |2021年第9期|129899.1-129899.8|共8页
  • 作者

    Mingyuan Zou; Feiya Su; Rui Zhang; Xinglu Jiang; Han Xiao; XueJiao Yan; Chuankun Yang; Xiaobo Fan; Guoqiu Wu;

  • 作者单位

    Medical School of Southeast University Nanjing 210009 People's Republic of China;

    Medical School of Southeast University Nanjing 210009 People's Republic of China;

    Medical School of Southeast University Nanjing 210009 People's Republic of China;

    Medical School of Southeast University Nanjing 210009 People's Republic of China;

    Medical School of Southeast University Nanjing 210009 People's Republic of China;

    The Affiliated Changzhou No. 2 People s Hospital of Nanjing Medical University Changzhou 213000 People's Republic of China;

    Center of Clinical Laboratory Medicine Zhongda Hospital Southeast University Nanjing 210009 People's Republic of China;

    Medical School of Southeast University Nanjing 210009 People's Republic of China;

    Medical School of Southeast University Nanjing 210009 People's Republic of China Center of Clinical Laboratory Medicine Zhongda Hospital Southeast University Nanjing 210009 People's Republic of China Jiangsu Provincial Key Laboratory of Critical Care Medicine Southeast University People's Republic of China;

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  • 正文语种 eng
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  • 关键词

    sars-cov-2; Nucleic acid test; Rapid diagnostic test; Catalytic hairpin assembly reaction; Lateral flow immunoassay strip;

    机译：SARS-CoV-2;核酸试验;快速诊断测试;催化发夹组装反应;横向流动免疫测定条;

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