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首页> 外文期刊>Sensors and Actuators >A gold nanoparticle based immunosorbent bio-barcode assay combined with real-time immuno-PCR for the detection of polychlorinated biphenyls
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A gold nanoparticle based immunosorbent bio-barcode assay combined with real-time immuno-PCR for the detection of polychlorinated biphenyls

机译：一种基于金纳米颗粒的免疫吸附生物条形码检测技术，结合实时免疫PCR技术来检测多氯联苯

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摘要

A gold nanoparticle based bio-barcode assay combined with real-time immuno- polymerase chain reaction (PCR) was developed for the determination of polychlorinated biphenyls (PCBs). Gold nanoparticles modified with thiol-capped DNA and antibody for affixing probes were used to replace antibody-DNA conjugates in immuno-PCR. The modified gold nanoparticles were fixed on the PCR tube wall through a competitive immune reaction. Signal-DNA could be released from the gold nanoparticle surface into the tubes and used as template DNA. Real-time PCR was subsequently performed to directly quantify DNA signal. A detection limit of 2.55 pg L~(-1) was obtained under optimized conditions. This method was successfully used to detect Aroclor 1248 in Hairtail samples collected from the East China Sea near Zhejiang Province. Recovery values ranged from 84% to 104%, and the coefficient of variation (CV) was acceptable. Results were validated by GC-ECD, indicating that this convenient and sensitive method has great potential for trace PCBs screening in real environmental samples.
机译：结合实时免疫聚合酶链反应（PCR）的金纳米颗粒生物条形码测定法被开发出来，用于测定多氯联苯（PCBs）。用巯基封端的DNA和抗体修饰的金纳米颗粒用于固定探针，用于取代免疫PCR中的抗体-DNA结合物。修饰的金纳米颗粒通过竞争性免疫反应固定在PCR管壁上。信号DNA可以从金纳米颗粒表面释放到试管中，并用作模板DNA。随后进行实时PCR以直接定量DNA信号。在优化条件下的检出限为2.55 pg L〜（-1）。该方法已成功用于检测浙江省附近东海采集的带毛样品中的Aroclor 1248。回收率范围从84％到104％，变异系数（CV）是可以接受的。 GC-ECD验证了结果，表明该便捷，灵敏的方法在实际环境样品中进行痕量PCB筛选方面具有巨大潜力。

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  • 来源
    《Sensors and Actuators》 |2015年第7期|152-158|共7页
  • 作者

    Guangxin Yang; Huisheng Zhuang; Hanyu Chen; Xianyin Ping; Dan Bu;

  • 作者单位

    School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China,Chinese Academy of Fishery Sciences, East China Sea Fisheries Research Institute, Aquatic Product Quality Inspection and Test Center (Shanghai), Laboratory of Quality & Safety Risk Assessment for Aquatic products (Shanghai), Ministry of Agriculture of P. R. China, Shanghai 200090, China;

    School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China;

    School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China;

    School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China,Chinese Academy of Fishery Sciences, East China Sea Fisheries Research Institute, Aquatic Product Quality Inspection and Test Center (Shanghai), Laboratory of Quality & Safety Risk Assessment for Aquatic products (Shanghai), Ministry of Agriculture of P. R. China, Shanghai 200090, China;

    School of Environmental Science and Engineering, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai, 200240, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Gold nanoparticle; PCBs; Real-time immuno-PCR; Signal DNA;

    机译：金纳米粒子;PCB;实时免疫PCR信号DNA;

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