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首页> 外文期刊>Sensors and Actuators >Evaluation of phage-based magnetoelastic biosensors for direct detection of Salmonella Typhimurium on spinach leaves
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Evaluation of phage-based magnetoelastic biosensors for direct detection of Salmonella Typhimurium on spinach leaves

机译：基于噬菌体的磁弹性生物传感器直接检测菠菜中鼠伤寒沙门氏菌的评估

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For a phage-based magnetoelastic (ME) biosensor method to become accepted for evaluating foodborne contamination, the method of use must be objectively compared, evaluated and validated against more widely accepted standard methods. In this study, a ME biosensor method was evaluated by comparison with TaqMan-based quantitative PCR (qPCR) for the detection of Salmonella Typhimurium on spinach leaves. Limit of detection (LOD) was determined after serial inoculation of S. Typhimurium on spinach leaves. The LOD for the ME biosensor was 2.17 and 1.94 log CFU/spinach for adaxial and abaxial surface, respectively. The LOD for qPCR was 1.37 log CFU/spinach. The repeatability of both methods was measured over a three day period by inoculation of S. Typhimurium on spinach leaves. The repeatability of the ME biosensor method was determined to be 6.38%, competitive with qPCR at 1.92%. For the direct comparison of both methods, 3 log CFU/spinach of S. Typhimurium was grown on two groups of 25 spinach leaves for 24 h and the number of S. Typhimurium was quantified by both methods. After confirmation of the growth, S. Typhimurium was positively detected and the quantified numbers were 5.79 ± 0.88 and 6.11 ± 0.26 log CFU/spinach for the ME biosensor and the qPCR method, respectively. This study demonstrated that the ME biosensor method was competitive and promising as an on-site and in-field detection method for the detection of pathogens.
机译：为了使基于噬菌体的磁弹性（ME）生物传感器方法能够用于评估食源性污染，必须对照更广泛接受的标准方法对使用方法进行客观比较，评估和验证。在这项研究中，通过与基于TaqMan的定量PCR（qPCR）进行比较，评估了一种ME生物传感器方法，用于检测菠菜叶上的鼠伤寒沙门氏菌。在菠菜叶上连续接种鼠伤寒沙门氏菌后确定检测限（LOD）。 ME生物传感器的LOD分别为正面和背面的log为2.17和1.94 log CFU /菠菜。 qPCR的LOD为1.37 log CFU /菠菜。通过在菠菜叶上接种鼠伤寒沙门氏菌，在三天的时间内测量了这两种方法的可重复性。经测定，ME生物传感器方法的重复性为6.38％，与qPCR的竞争性为1.92％。为了直接比较这两种方法，将鼠伤寒沙门氏菌的3 log CFU /菠菜在两组25张菠菜叶片上生长24小时，并通过两种方法对鼠伤寒沙门氏菌的数量进行定量。确认生长后，阳性检出鼠伤寒沙门氏菌，ME生物传感器和qPCR方法的定量数分别为5.79±0.88和6.11±0.26 log CFU /菠菜。这项研究表明，ME生物传感器方法具有竞争性，并有望作为一种用于病原体检测的现场和现场检测方法。

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