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首页> 外文期刊>Sensors and Actuators >Detection of glyphosate by quantitative analysis of fluorescence and single DNA using DNA-labeled fluorescent magnetic core-shell nanoparticles
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Detection of glyphosate by quantitative analysis of fluorescence and single DNA using DNA-labeled fluorescent magnetic core-shell nanoparticles

机译：通过使用DNA标记的荧光磁性核壳纳米粒子对荧光和单个DNA进行定量分析来检测草甘膦

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摘要

Biosensors based on antibody recognition have a wide range of monitoring applications with applications to environmental and food problems. In an effort to improve the limit of detection, novel Co-B/SiO2/dye nanoparticles were prepared in a water-in-oil microemulsion and subsequently surface modified. The fluorescence magnetic nanoparticle (FMP) based detection method for quantitatively fluorescence intensity and specific DNA-based application is described. A sandwich format consisting of conjugated glyphosate-double target/probe DNA-FMP was fabricated. Results were measured based on the fluorescence intensity obtained by comparing the free glyphosates at concentrations of 1-10,000 nM for detecting the binding of glyphosate-double DNA-FMP nanoparticles. Moreover, quantitative information concerning the free glyphosate analysis was translated into a single probe DNA signal with a good linear correlation in the concentration range of 1-10,000 nM (R~2 =0.98) and a limit of detection of 0.27 nM. Therefore, detection of glyphosate could easily and quantitatively be measured using confocal laser scanning microscopy and low-cost UV photometric analysis. These results indicate that this method is a very simple and economic strategy for detecting glyphosate.
机译：基于抗体识别的生物传感器在环境和食品问题方面具有广泛的监控应用。为了提高检测限，在油包水微乳液中制备了新型Co-B / SiO2 /染料纳米颗粒，然后对其进行了表面改性。描述了用于定量荧光强度的基于荧光磁性纳米颗粒（FMP）的检测方法和基于特定DNA的应用。制备了由草甘膦-双靶/探针DNA-FMP共轭组成的三明治格式。基于荧光强度测量结果，该荧光强度通过比较浓度为1-10,000 nM的游离草甘膦用于检测草甘膦-双DNA-FMP纳米颗粒的结合而获得。此外，有关游离草甘膦分析的定量信息被转换为单一探针DNA信号，在1-10,000 nM（R〜2 = 0.98）的浓度范围内且检测限为0.27 nM，具有良好的线性相关性。因此，使用共聚焦激光扫描显微镜和低成本的紫外光度分析可以轻松，定量地检测草甘膦的含量。这些结果表明，该方法是检测草甘膦的一种非常简单且经济的方法。

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  • 来源
    《Sensors and Actuators》 |2013年第2期|879-886|共8页
  • 作者

    Hee Uk Lee; Da UnJung; Ja Hyun Lee; Yoon Seok Song; Chulhwan Park; Seung Wook Kim;

  • 作者单位

    Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Republic of Korea;

    Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Republic of Korea;

    Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Republic of Korea;

    Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Republic of Korea;

    Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-Dong, Nowon-Gu, Seoul 139-701, Republic of Korea;

    Department of Chemical and Biological Engineering, Korea University, 5 Ga, Anam-Dong, Sungbuk-Gu, Seoul 136-701, Republic of Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    immunosensor; glyphosate; fluorescence; quantitative analysis; water-in-oil; magnetic nanoparticle;

    机译：免疫传感器草甘膦荧光定量分析;油包水;磁性纳米粒子;

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